Human Granzyme B ELISPOT Ready-SET-Go!®

Also known as: GrzB, GrB

RUO: For Research Use Only

human/mouse Granzyme B antibody, Cytokine Kits conjugate

Normal human peripheral blood cells unstimulated (left) or stimulatede with PMA/Ionomycin for 24 hrs (right) in the Human Granzyme B ELISPOT Assay

Contents: Human Granzyme B ELISPOT Ready-SET-Go!®
Catalog Number: 88-8399
Storage Conditions: Store at 2-8°C.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
88-8399-21	2 plates	To view relevant pricing, please select your country and resume browsing.
88-8399-88	10 plates

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-8822	Anti-Mouse Granzyme B FITC (16G6)
12-8822	Anti-Mouse Granzyme B PE (16G6)
12-8899	Phycoerythrin (PE) anti-human Granzyme B (GB11)
13-8822	Anti-Mouse Granzyme B Biotin (16G6)
14-8822	Anti-Mouse Granzyme B Purified (16G6)
88-8022	Mouse Granzyme B ELISA Ready-SET-Go!®

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-8399	Human Granzyme B ELISPOT Ready-SET-Go!®	N/A	N/A	ELISPOT	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

One of the major mechanisms of cell-mediated cytotoxicity involves exocytosis of cytoplasmic granules from the effector toward the target cell. The granules contain a number of proteins, including the pore-forming protein perforin and a family of serine proteases called granzymes, including Granzyme B. Granzyme B is present mainly in the granules of CD8+ CTL and natural killer (NK) cells and mediates the lethal hit that kills virus infected and tumorigenic cells. Measurement of release of Granzyme B in response to the appropriate target is useful for evaluating cell-mediated cytotoxicity. The Granzyme B ELISPOT assay is a valuable non-radioactive alternative to the 51Cr-release killing cell assays for measuring antigen-specific CTL cytotoxicity.

This Granzyme B ELISPOT Ready-SET-Go! reagent set contains the necessary reagents for performing enzyme linked immunosorbent spot (ELISPOT) assays for high resolution frequency analysis of Granzyme B-secreting cells. This ELISPOT reagent set is pre-titrated for optimal spot development.

Components

Capture Antibody. Pre-titrated, Functional Grade (low endotoxin) purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
ELISA/ELISPOT Coating Buffer Powder. Reconstitute to 1L with dH20 and filter (0.22 uM).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme

Applications Reported

For research use only, not for diagnostic or therapeutic use.

References

Shafer-Weaver, K., et al. 2003. The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity. J. Translational Med. 1: 14.
Rininsland, F., et al. 2000. Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. J Immunol Meth. 240:143-155.

Protocol

To view protocol, please download the TDS by clicking the PDF button above.

Copyright © 2000-2010 eBioscience, Inc.
Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.