Human Granzyme B ELISPOT Ready-SET-Go!

RUO: For Research Use Only

mouse/human Granzyme B antibody, Cytokine Kits conjugate

Human Granzyme B ELISPOT Assay. Left: Human PBMCs without mitogen cultured for 24 hrs. Right: Human PBMCs activated with PMA/Ionomycin for 24 hrs.

Contents: Human Granzyme B ELISPOT Ready-SET-Go!
Catalog Number: 88-8399

Product Options
Cat. No.	Size	Price	Add Qty to Cart
88-8399-21	2 plates	To view relevant pricing, please select your country and resume browsing.
88-8399-88	10 plates

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

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12-8822	Phycoerythrin (PE) anti-mouse Granzyme B (16G6)
12-8899	Phycoerythrin (PE) anti-human Granzyme B (GB11)
13-8822	Biotin anti-mouse Granzyme B (16G6)
14-8822	Affinity Purified anti-mouse Granzyme B (16G6)
88-8022	Mouse Granzyme B ELISA Ready-SET-Go!

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-8399	Human Granzyme B ELISPOT Ready-SET-Go!	N/A	N/A	ELISPOT	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

One of the major mechanisms of cell-mediated cytotoxicity involves exocytosis of cytoplasmic granules from the effector toward the target cell. The granules contain a number of proteins, including the pore-forming protein perforin and a family of serine proteases called granzymes, including Granzyme B. Granzyme B is present mainly in the granules of CD8+ CTL and natural killer (NK) cells and mediates the lethal hit that kills virus infected and tumorigenic cells. Measurement of release of Granzyme B in response to the appropriate target is useful for evaluating cell-mediated cytotoxicity. The Granzyme B ELISPOT assay is a valuable non-radioactive alternative to the 51Cr-release killing cell assays for measuring antigen-specific CTL cytotoxicity.

This Granzyme B ELISPOT Ready-SET-Go! reagent set contains the necessary reagents for performing enzyme linked immunosorbent spot (ELISPOT) assays for high resolution frequency analysis of Granzyme B-secreting cells. This ELISPOT reagent set is pre-titrated for optimal spot development.

Components

Capture Antibody. Pre-titrated, Functional Grade (low endotoxin) purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
ELISA/ELISPOT Coating Buffer Powder. Reconstitute to 1L with dH20 and filter (0.22 uM).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme

Applications Reported

For research use only, not for diagnostic or therapeutic use.

References

Shafer-Weaver, K., et al. 2003. The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity. J. Translational Med. 1: 14.
Rininsland, F., et al. 2000. Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. J Immunol Meth. 240:143-155.

Protocol

To view protocol, please download the TDS by clicking the PDF button above.