Normal human peripheral blood cells were cultured with PMA (50 ng/ml), ionomycin (500 ng/ml), and LPS (1 µg/ml) for 24 hours in a Human IL-10 ELISPOT assay (right). Left well is medium alone (no mitogen).
Contents: Human IL-10 ELISPOT Ready-SET-Go!® Catalog Number: 88-7805 Storage Conditions: Store at 2-8°C.
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Size
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88-7805-21
2 plate
88-7805-88
10 plate
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This Human IL-10 ELISPOT Ready-SET-Go! reagent set contains the necessary reagents for performing enzyme linked immunosorbent spot (ELISPOT) assays for high resolution frequency analysis of IL-10-secreting cells. This ELISPOT reagent set is pre-titrated for optimal spot development.
Components
Capture Antibody. Pre-titrated, Functional Grade (low endotoxin) purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody ELISA/ELISPOT Coating Buffer Powder. Reconstitute to 1L with dH20 and filter (0.22 uM). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Certificate of Analysis. Lot-specific instructions for dilution of antibodies and enzyme
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Protocol
To view protocol, please download the TDS by clicking the PDF button above.
