Human Th1/Th2 ELISA Ready-SET-Go!®

Also known as: IFNg, IL-10, IL-2, IL-4

RUO: For Research Use Only

Contents: Human Th1/Th2 ELISA Ready-SET-Go!®
Catalog Number: 88-7799
Sensitivity:
IFN-gamma: 4 pg/ml
IL-2: 4 pg/ml
IL-4: 2 pg/ml
IL-10: 2 pg/ml

Standard Curve Range:
IFN-gamma: 4 - 500 pg/ml
IL-2: 4 - 500 pg/ml
IL-4: 2 - 200 pg/ml
IL-10: 2 - 300 pg/ml

Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
88-7799-44	Panel	To view relevant pricing, please select your country and resume browsing.

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-7799	Human Th1/Th2 ELISA Ready-SET-Go!®	N/A	N/A	ELISA	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The eBioscience Human Th1/Th2 ELISA Ready-SET-Go! contains 4 pairs of antibodies that can be used to discriminate Th1/Th2 subsets based on their expression of IFN-γ, IL-2, IL-4 and IL-10. This reagent panel contains all of the necessary antibody reagents, standards, avidin-HRP, TMB substrate solution, coating buffers and diluents for performing two 96-well ELISA plate assays for each of these four cytokines. This ELISA reagent set is specifically engineered for accurate and precise measurement of cytokine protein levels from samples including serum, plasma, and supernatants from cell cultures.

Components

Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. Reconstitute to 1L with dH20 and filter (0.22 uM).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included)

References

Baba M, Yong Ma B, Nonaka M, Matsuishi Y, Hirano M, Nakamura N, Kawasaki N, Kawasaki N, Kawasaki T. Glycosylation-dependent interaction of Jacalin with CD45 induces T lymphocyte activation and Th1/Th2 cytokine secretion. J Leukoc Biol. 2007 Apr;81(4):1002-11. (RSG ELISA kit, TC supernatant, PubMed)

Atègbo JM, Grissa O, Yessoufou A, Hichami A, Dramane KL, Moutairou K, Miled A, Grissa A, Jerbi M, Tabka Z, Khan NA. Modulation of adipokines and cytokines in gestational diabetes and macrosomia. J Clin Endocrinol Metab. 2006 Oct;91(10):4137-43. (RSG ELISA kit, serum, PubMed)

Protocol

To view protocol, please download the TDS by clicking the PDF button above.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.