Human/Mouse TGFb1 ELISA Ready-SET-Go! Kit (Discontinued. Please see replacement item BMS249/3)

RUO: For Research Use Only

human/mouse TGF-beta-1 antibody, Cytokine Kits conjugate

Four parameter fit of Human/mouse TGF-b1 ELISA Ready-SET-Go! Recombinant concentration in pg/ml.

Contents: Human/Mouse TGFb1 ELISA Ready-SET-Go! Kit (Discontinued. Please see replacement item BMS249/3)
Catalog Number: 88-7449
Sensitivity: 60 pg/ml
Standard Curve Range: 60 pg/ml - 8 ng/ml

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Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
BMS249/3	Human TGF-beta 1 Platinum ELISA
BMS249/3TEN	Human TGF-beta 1 Platinum ELISA

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-7449	DISCONTINUED - Human/ Mouse TGFb1 ELISA Ready-SET-Go! Kit (See replacement item BMS249/3)	N/A	N/A	ELISAkit	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

Transforming Growth Factor-beta (TGF-β) is a pleiotropic cytokine which exists in five isoforms, known as TGF-β1-5, with homologies of 70-80% and no homology to TGF-α. TGF-β1 is the most abundant form in lymphoid organs and is found almost ubiquitously while other isoforms are expressed in a more restricted distribution. The biologically active forms of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The isoforms of TGF-β arise by proteolytic cleavage of longer precursors; the isoforms are derived from the carboxyterminal ends of these precursors. Isoforms isolated from different species are evolutionarily closely conserved and have sequence identities on the order of 98%. Mature human, porcine, simian, chicken and bovine TGF-β1 are identical and differ from mouse TGF-β1 in a single amino acid. TGF-β1 is produced in very high levels by platelets. Other cellular sources of TGF-β1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-β1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-β1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-β1 inhibits the proliferation of T cells and NK cells and downregulates the activities of activated macrophages. TGF-β1 blocks the anti-tumor activity of IL-2 – bearing lymphokine-activated killer (LAK) cells.

Recently, TGF-β1 has been found to have a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-β1 and stimulate expansion of naturally-occurring T reg cells. Moreover, TGF-β1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-β-induced regulatory T cells have been termed Ti-Treg.

This Human/Mouse TGF-β1 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates) contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for accurate and precise measurement of mouse or human TGF-β1 protein levels from samples including serum, plasma, and supernatants from cell cultures.

Note: This sandwich ELISA recognizes the mature/active form of TGF-β1. Samples (but not standards) should be acid-treated and then neutralized to activate the latent TGF-β1 to the immunoreactive form. Samples should be tested in the ELISA immediately after acid treatment and neutralization. See 'Experimental Procedure'.

Components

Pre-coated ELISA Plate
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
Assay Diluent. 5X concentrated
ELISA Wash Buffer Powder. Reconstitute in 1 L dI water
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Stop Solution. 5 mls of 1X solution per plate
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards

Applications Reported

For research use only, not for diagnostic or therapeutic use.

References

Miyazono, K., et al. 1992. Structure, function and possible clinical application of transforming growth factor-beta. J Dermatol. 19: 644-647.
Roberts, A., et al. 1993. Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta). Growth Factors 8: 1-9.
Peng, Y., et al. 2004. TGF-beta regulates in vivo expansion of Foxp3-expressing CD4+CD25+ regulatory T cells responsible for protection against diabetes. PNAS. 101: 4572-4577.
Fantini, M., et al. 2005. TGF-beta – induced Foxp3+ regulatory T cells suppress Th1-mediated experimental colitis. Gut. [Epub ahead of print]
Zheng, S.G., et al. 2002. Generation ex vivo of TGF-b-producing regulatory T cells from CD4+CD25- precursors. J. Immunol. 169: 4183-4189.

Protocol

To view protocol, please download the TDS by clicking the PDF button above.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.