Also known as: Tumor Necrosis Factor alpha, TNFa TNF-a
RUO: For Research Use Only
Standard curve of Human TNa ELISA Ready-SET-Go!®
Contents: Human TNF alpha ELISA Ready-SET-Go!® Catalog Number: 88-7346 Sensitivity: 4 pg/ml Standard Curve Range: 4 - 500 pg/ml Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.
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88-7346-22
2 plates (includes plates)
88-7346-76
20 plates (includes plates)
88-7346-77
20 plates
88-7346-86
10 plates (includes plates)
88-7346-88
10 plates
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This Human TNF-α ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of human TNF-α protein levels from samples including serum, plasma, and supernatants from cell cultures.
Components
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
References
Marseille-Tremblay C, Ethier-Chiasson M, Forest JC, Giguère Y, Masse A, Mounier C, Lafond J. Impact of maternal circulating cholesterol and gestational diabetes mellitus on lipid metabolism in human term placenta. Mol Reprod Dev. 2008 Jun;75(6):1054-62. (RSG ELISA kit, tissue homogenate, PubMed)
Tversky JR, Le TV, Bieneman AP, Chichester KL, Hamilton RG, Schroeder JT. Human blood dendritic cells from allergic subjects have impaired capacity to produce interferon-alpha via Toll-like receptor 9. Clin Exp Allergy. 2008 May;38(5):781-8. (RSG ELISA kit, TC supernatant, PubMed)
Ahuja J, Lepoutre V, Wigdahl B, Khan ZK, Jain P. Induction of pro-inflammatory cytokines by human T-cell leukemia virus type-1 Tax protein as determined by multiplexed cytokine protein array analyses of human dendritic cells. Biomed Pharmacother. 2007 May;61(4):201-8. (RSG ELISA kit, TC supernatant, PubMed)
Utz JP, Limper AH, Kalra S, Specks U, Scott JP, Vuk-Pavlovic Z, Schroeder DR. Etanercept for the treatment of stage II and III progressive pulmonary sarcoidosis. Chest. 2003 Jul;124(1):177-85. (RSG ELISA kit, TC supernatant, serum, and lavage fluid, PubMed)
Protocol
To view protocol, please download the TDS by clicking the PDF button above.
