Human/Mouse TGF beta 1 ELISA Ready-SET-Go!®

Also known as: Transforming Growth Factor beta 1, TGFbeta1, TGF-b1

RUO: For Research Use Only

human/mouse TGF-beta-1 antibody, Cytokine Kits conjugate

Standard curve of Human/Mouse TGFβ ELISA Ready-SET-Go!®

Contents: Human/Mouse TGF beta 1 ELISA Ready-SET-Go!®
Catalog Number: 88-7344
Sensitivity: 60 pg/ml
Standard Curve Range: 60 pg/ml - 8000 pg/ml
Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
88-7344-22	2 plates (includes plates)	To view relevant pricing, please select your country and resume browsing.
88-7344-76	20 plates (includes plates)
88-7344-77	20 plates
88-7344-86	10 plates (includes plates)
88-7344-88	10 plates

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-7344	Human/Mouse TGF beta 1 ELISA Ready-SET-Go!®	N/A	N/A	ELISAset	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

Transforming Growth Factor-beta (TGF-β) is a pleiotropic cytokine which exists in five isoforms, known as TGF-β1-5, with homologies of 70-80% and no homology to TGF-α. TGF-β1 is the most abundant form in lymphoid organs and is found almost ubiquitously while other isoforms are expressed in a more restricted distribution. The biologically active forms of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The isoforms of TGF-β arise by proteolytic cleavage of longer precursors; the isoforms are derived from the carboxyterminal ends of these precursors. Isoforms isolated from different species are evolutionarily closely conserved and have sequence identities on the order of 98%. Mature human, porcine, simian, chicken and bovine TGF-β1 are identical and differ from mouse TGF-β1 in a single amino acid. TGF-β1 is produced in very high levels by platelets. Other cellular sources of TGF-β1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-β1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-β1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-β1 inhibits the proliferation of T cells and NK cells and downregulates the activities of activated macrophages. TGF-β1 blocks the anti-tumor activity of IL-2 – bearing lymphokine-activated killer (LAK) cells.

Recently, TGF-β1 has been found to have a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-β1 and stimulate expansion of naturally-occurring T reg cells. Moreover, TGF-β1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-β-induced regulatory T cells have been termed Ti-Treg.

This Mouse/Human TGF-β1 Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for accurate and precise measurement of mouse or human TGF-β1 protein levels from samples including serum, plasma, and supernatants from cell cultures. The calibration standard is highly purified, bioactive recombinant human TGF-β1 (mature form).

Note: This sandwich ELISA recognizes the mature/active form of TGF-β1. Samples (but not standards) should be acid-treated and then neutralized to activate the latent TGF-β1 to the immunoreactive form. Samples should be tested in the ELISA immediately after acid treatment and neutralization. See 'Experimental Procedure'.

Components

Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)

Applications Reported

For research use only, not for diagnostic or therapeutic use.

References

Luque J, Lozano JM, García-Jurado G, Soriano-Sarabia N, González R, Vallejo A, Leal M, Peña J. NK-associated regulatory receptors in a structured HAART interruption of HIV-1-positive individuals. AIDS Res Hum Retroviruses. 2008 Aug;24(8):1037-42. (RSG ELISA kit, human plasma, PubMed)

Mukherjee S, Ahmed A, Nandi D. CTLA4-CD80/CD86 interactions on primary mouse CD4+ T cells integrate signal-strength information to modulate activation with Concanavalin A. J Leukoc Biol. 2005 Jul;78(1):144-57. (RSG ELISA kit, TC supernatant, PubMed)

Lee SI, Hyun PM, Kim SH, Kim KS, Lee SK, Kim BS, Maeng PJ, Lim JS. Suppression of the onset and progression of collagen-induced arthritis by chebulagic acid screened from a natural product library. Arthritis Rheum. 2005 Jan;52(1):345-53. (RSG ELISA kit, mouse serum, PubMed)

Peng Y, Laouar Y, Li MO, Green EA, Flavell RA. TGF-beta regulates in vivo expansion of Foxp3-expressing CD4+CD25+ regulatory T cells responsible for protection against diabetes. Proc Natl Acad Sci U S A. 2004 Mar 30;101(13):4572-7.

Roberts AB, Sporn MB. Physiological actions and clinical applications of transforming growth factor-beta (TGF-beta). Growth Factors. 1993;8(1):1-9.

Miyazono K, Heldin CH. Structure, function and possible clinical application of transforming growth factor-beta. J Dermatol. 1992 Nov;19(11):644-7.

Fantini MC, Becker C, Tubbe I, Nikolaev A, Lehr HA, Galle P, Neurath MF. TGF-beta – induced Foxp3+ regulatory T cells suppress Th1-mediated experimental colitis. Gut. 2006 May;55(5):671-80.

Protocol

To view protocol, please download the TDS by clicking the PDF button above.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.