Also known as: TNF a, TNF alpha, TNFalpha, Tumor necrosis factor alpha
RUO: For Research Use Only
Standard curve of Rat TNFa ELISA Ready-SET-Go!®
Contents: Rat TNF alpha ELISA Ready-SET-Go!® Catalog Number: 88-7340 Sensitivity: 16 pg/ml Standard Curve Range: 16 pg/ml - 2000 pg/ml Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.
Product Options
Cat. No.
Size
Price
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88-7340-22
2 plates (includes plates)
88-7340-76
20 plates (includes plates)
88-7340-77
20 plate
88-7340-86
10 plates (includes plates)
88-7340-88
10 plate
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The Rat TNF-α ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of rat TNF-α protein levels from samples including serum, plasma, and supernatants from cell cultures.
Components
Capture Antibody. Pre-titrated, purified antibody Detection Antibody. Pre-titrated, biotin-conjugated antibody Standard. Recombinant cytokine for generating standard curve and calibrating samples ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20). Assay Diluent. 5X concentrated Detection enzyme. Pre-titrated Avidin-HRP Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards 96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)
Applications Reported
For research use only, not for diagnostic or therapeutic use.
References
Lehmann GL, Carreras FI, Soria LR, Gradilone SA, Marinelli RA. LPS induces the TNF-alpha-mediated downregulation of rat liver aquaporin-8: role in sepsis-associated cholestasis. Am J Physiol Gastrointest Liver Physiol. 2008 Feb;294(2):G567-75. (RSG ELISA, serum, PubMed)
Zhang W, Lu C, et al. 2007. Therapeutic effect of combined triptolide and glycyrrhizin treatment on rats with collagen induced arthritis. Planta Med. 73(4):336-40. (RSG ELISA, serum, PubMed)
Zeng Y, Gu B, et al. 2007. Sinomenine, an antirheumatic alkaloid, ameliorates clinical signs of disease in the Lewis rat model of acute experimental autoimmune encephalolmyelitis. Biol Pharm Bull. 30(8):1438-44. (RSG ELISA, TC supernatant, PubMed)
Protocol
To view protocol, please download the TDS by clicking the PDF button above.
