Mouse TNF alpha ELISA Ready-SET-Go!®

Also known as: Tumor Necrosis Factor alpha, TNFa TNF-a

RUO: For Research Use Only

Mouse TNFa ELISA Kit: Mouse Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) ELISA Ready-SET-Go!

Standard curve of Mouse TNFα ELISA Ready-SET-Go!®

Contents: Mouse TNF alpha ELISA Ready-SET-Go!®
Catalog Number: 88-7324
Sensitivity: 8 pg/ml
Standard Curve Range: 8 - 1000 pg/ml
Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
88-7324-22	2 plates (includes plates)	To view relevant pricing, please select your country and resume browsing.
88-7324-76	20 plates (includes plates)
88-7324-77	20 plates
88-7324-86	10 plates (includes plates)
88-7324-88	10 plates

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
88-7013	Mouse IL-1 beta ELISA Ready-SET-Go!®
88-7121	Mouse IL-12 p70 ELISA Ready-SET-Go!®
88-7234	Mouse IL-23 ELISA Ready-SET-Go!®
88-8314	Mouse IFNg ‘Femto-HS’ High Sensitivity ELISA Ready-Set-Go!

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-7324	Mouse TNF alpha ELISA Ready-SET-Go!®	N/A	N/A	ELISAset	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

This Mouse TNF-α ELISA Ready-SET-Go! reagent set (with or without high-affinity binding microwell plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse TNF-α protein levels from samples including serum, plasma, and supernatants from cell cultures. Recognition of mouse TNF-α by this assay is not interfered with by 1000x excess soluble TNF-α receptor type I or II.

Components

Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)

References

Cho HJ, Seon MR, Lee YM, Kim J, Kim JK, Kim SG, Park JH. 3,3'-Diindolylmethane suppresses the inflammatory response to lipopolysaccharide in murine macrophages. J Nutr. 2008 Jan;138(1):17-23. (RSG ELISA kit, TC supernatant, PubMed)

Nakano T, Araki K, Nakatani H, Kobayashi M, Sugimoto T, Furuya Y, Matsuoka T, Jin T, Hanazaki K. Effects of geldanamycin and thalidomide on the Th1/Th2 cytokine balance in mice subjected to operative trauma. Surgery. 2007 Apr;141(4):490-500. (RSG ELISA kit, serum, PubMed)

Chen L, Lin SX, Agha-Majzoub R, Overbergh L, Mathieu C, Chan LS. CCL27 is a critical factor for the development of atopic dermatitis in the keratin-14 IL-4 transgenic mouse model. Int Immunol. 2006 Aug;18(8):1233-42. (RSG ELISA kit, tissue homogenate, PubMed)

Yang D, Zhang Y, Nguyen HG, Koupenova M, Chauhan AK, Makitalo M, Jones MR, St Hilaire C, Seldin DC, Toselli P, Lamperti E, Schreiber BM, Gavras H, Wagner DD, Ravid K. The A2B adenosine receptor protects against inflammation and excessive vascular adhesion. J Clin Invest. 2006 Jul;116(7):1913-23. (RSG ELISA kit, plasma, PubMed)

Mallen-St Clair J, Pham CT, Villalta SA, Caughey GH, Wolters PJ. Mast cell dipeptidyl peptidase I mediates survival from sepsis. J Clin Invest. 2004 Feb;113(4):628-34. (RSG ELISA kit, lavage fluid, PubMed)

Protocol

To view protocol, please download the TDS by clicking the PDF button above.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.