Human IL-23 ELISA Ready-SET-Go!® Set

Also known as: Interleukin-23, IL23, p40, p19

RUO: For Research Use Only

human IL-23 antibody, Cytokine Kits conjugate

Standard curve of Human IL-23 ELISA Ready-SET-Go!®

Contents: Human IL-23 ELISA Ready-SET-Go!® Set
Catalog Number: 88-7237
Sensitivity: 15 pg/ml
Standard Curve Range: 15 pg/ml - 2.0 ng/ml
Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
88-7237-22	2 plates (includes plates)	To view relevant pricing, please select your country and resume browsing.
88-7237-86	10 plates (includes plates)
88-7237-88	10 plates

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

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Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-7237	Human IL-23 ELISA Ready-SET-Go!® Set	N/A	N/A	ELISAset	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

This Human IL-23 Ready-SET-Go! ELISA Set contains the necessary reagents, standards, buffers and diluents for performing quantitative enzyme-linked immunosorbent assays (ELISA). This ELISA set is specifically engineered for accurate and precise measurement of human IL-23 protein levels from samples including serum, plasma, and supernatants from cell cultures. Interference with CpG has been observed; therefore controls must be added if using this compoound in the assay. The assay demonstrates parallelism in measuring recombinant and native human IL-23 proteins with a standard curve range of 15 pg/ml to 2,000 pg/ml, and assay sensitivity below 15 pg/ml. The assay has been validated by specific detection of significant levels of native human IL-23 protein in supernatants from a variety of different activated dendritic cell populations. The use of a p19-specific capture antibody and a p40-specific detection antibody renders this sandwich ELISA exquisitely specific for human IL-23. IL-12 p40 monomer and IL-12 p70 were run in the assay at 200 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was also run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed.

IL-23 is a heterodimeric cytokine composed of the p40 subunit of IL-12 disulfide-linked with a protein p19. p19, like p35 of IL-12, is biologically inactive by itself. IL-23 interacts with IL-12Rbeta1 and an additional, novel beta2-like receptor subunit with STAT4 binding domain, termed IL-23R. IL-23 is secreted by activated mouse and human dendritic cells. Biological activities of mouse IL-23 are distinct from those of mouse IL-12. Mouse IL-23 was found not to induce significant amounts of IFN-g. Mouse IL-23 does induce strong proliferation of memory T cells (but not naïve T cells), whereas IL-12 has no effect on memory cells. Additionally, mouse IL-23 (but not IL-12) can activate mouse memory T cells to produce the proinflammatory cytokine IL-17. Human IL-23 has biological properties which are less distinct from human IL-12; human IL-23 induces proliferation of memory T cells and induces moderate levels of IFN-g production by naïve and memory T cells, as compared to IL-12. IL-23-dependent, IL-17-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. More recently, de novo differentiation of Th-17 cells in the absence of IL-23 has been demonstrated by treatment of naïve CD4 cells with TGF-β1 and IL-6.

Components

Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples
ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plate. Corning Costar 9018 (included with product Cat. #’s ending in suffixes -22, -44, -76, -86)

Applications Reported

For research use only, not for diagnostic or therapeutic use.

References

Goyarts E, Matsui M, Mammone T, Bender AM, Wagner JA, Maes D, Granstein RD. Norepinephrine modulates human dendritic cell activation by altering cytokine release. Exp Dermatol. 2008 Mar;17(3):188-96. (RSG ELISA kit, TC supernatant, PubMed)

Enstrom A, Onore C, Hertz-Picciotto I, Hansen R, Croen L, Van de WaterJ, Ashwood P. Detection of IL-17 and IL-23 in Plasma Samples of Children with Autism. American Journal of Biochemistry and Biotechnology 4 (2): 114-120, 2008. (RSG ELISA kit, plasma)

Iwakura Y, Ishigame H. The IL-23/IL-17 axis in inflammation. J Clin Invest. 2006 May;116(5):1218-22.

Veldhoen M, Hocking RJ, Atkins CJ, Locksley RM, Stockinger B. TGFbeta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17-producing T cells. Immunity. 2006 Feb;24(2):179-89.

Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, Murphy KM, Weaver CT. Interleukin 17–producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol. 2005 Nov;6(11):1123-32.

Brombacher F, Kastelein RA, Alber G. Novel IL-12 family members shed light on the orchestration of Th1 responses. Trends Immunol. 2003 Apr;24(4):207-12.

Aggarwal S, Ghilardi N, Xie MH, de Sauvage FJ, Gurney AL. IL-23 promotes a distinct CD4 T cell activation state characterized by the production of IL-17. J. J Biol Chem. 2003 Jan 17;278(3):1910-4.

Oppmann B, Lesley R, Blom B, Timans JC, Xu Y, Hunte B, Vega F, Yu N, Wang J, Singh K, Zonin F, Vaisberg E, Churakova T, Liu M, Gorman D, Wagner J, Zurawski S, Liu Y, Abrams JS, Moore KW, Rennick D, de Waal-Malefyt R, Hannum C, Bazan JF, Kastelein RA. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity. 2000 Nov;13(5):715-25.

Protocol

To view protocol, please download the TDS by clicking the PDF button above.

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