Mouse IL-23 ELISA Ready-SET-Go!®

Also known as: Interleukin-23, IL23, p40, p19

RUO: For Research Use Only

Contents: Mouse IL-23 ELISA Ready-SET-Go!®
Catalog Number: 88-7234
Sensitivity: 30 pg/ml
Standard Curve Range: 30 pg/ml - 4000 pg/ml
Storage Conditions: Store at 2-8°C except standard which should be stored at less than or equal to -70°C.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
88-7234-22	2 plates (includes plates)	To view relevant pricing, please select your country and resume browsing.
88-7234-86	10 plates (includes plates)
88-7234-88	10 plates

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
14-8231	Mouse IL-23 Recombinant Protein
16-7123	Anti-Mouse IL-12/IL-23 p40 Functional Grade Purified (C17.8)
16-7232	Anti-Mouse IL-23 p19 Functional Grade Purified (G23-8)
34-8231	Mouse IL-23 Recombinant Protein Carrier-Free
88-7120	Mouse IL-12/IL-23 total p40 ELISA Ready-SET-Go!®
88-7231	Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
88-7921	Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go! Kit (See replacement item BMS6004)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
88-7234	Mouse IL-23 ELISA Ready-SET-Go!®	N/A	N/A	ELISAset	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

This Mouse IL-23 Ready-SET-Go! ELISA Set (with high affinity binding ELISA plates) contains the necessary reagents, buffers and diluents for performing quantitative, enzyme-linked immunosorbent assays (ELISA). This ELISA reagent set is specifically engineered for accurate and precise measurement of mouse IL-23 protein levels from samples including serum, plasma, and supernatants from cell cultures. The assay demonstrates parallelism in measuring recombinant and native mouse IL-23 proteins with a standard curve range of 30 pg/ml to 4,000 pg/ml and assay sensitivity below 30 pg/ml. Native mouse IL-23 was detected in supernatants from bone marrow-derived, LPS-activated dendritic cells. The use of a p19-specific capture antibody and a p40-specific detection antibody renders this assay exquisitely specific for mouse IL-23. IL-12 p40 homodimer and IL-12 p70 were each run in the assay at 500 ng/ml with no interference or cross-reactivity observed. A panel of 20 unrelated cytokines was run in the IL-23 ELISA at 100 ng/ml with no cross reactivity observed; all values were at the limit of detection of the assay.

For measurement of total p40 protein levels, the Mouse IL-12/23 Total p40 ELISA Ready-SET-Go! is available (88-7120).

Components

Capture Antibody. Pre-titrated, purified antibody
Detection Antibody. Pre-titrated, biotin-conjugated antibody
Standard. Recombinant cytokine for generating standard curve and calibrating samples

ELISA/ELISPOT Coating Buffer Powder. This Ready-Set-Go! ELISA Set may contain ELISA/ELISPOT Coating Buffer Powder (Reconstitute to 1L with dH20 and filter (0.22 uM)) or 10X PBS ELISA Coating Buffer (Dilute 1 part 10X Buffer into 9 parts dH20).
Assay Diluent. 5X concentrated
Detection enzyme. Pre-titrated Avidin-HRP
Substrate Solution. Tetramethylbenzidine (TMB) Substrate Solution
Certificate of Analysis. Lot-specific instructions for dilution of antibodies and standards
96 Well Plates. Corning Costar 9018

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Special Notes

To ensure optimal results from this ELISA Ready-SET-Go! Kit, please only use the components included in the set. Exchanging of components is not recommended as a change in signal may occur.

References

Xu J, Racke MK, Drew PD. Peroxisome proliferator-activated receptor-alpha agonist fenofibrate regulates IL-12 family cytokine expression in the CNS: relevance to multiple sclerosis. J Neurochem. 2007 Dec;103(5):1801-10.(RSG ELISA kit, TC supernatant, PubMed)

Mikols CL, Yan L, Norris JY, Russell TD, Khalifah AP, Hachem RR, Chakinala MM, Yusen RD, Castro M, Kuo E, Patterson GA, Mohanakumar T, Trulock EP, Walter MJ. IL-12 p80 is an innate epithelial cell effector that mediates chronic allograft dysfunction. Am J Respir Crit Care Med. 2006 Aug 15;174(4):461-70. (RSG ELISA kit, protein lysate, PubMed)

Cruz A, Khader SA, Torrado E, Fraga A, Pearl JE, Pedrosa J, Cooper AM, Castro AG. Cutting edge: IFN-gamma regulates the induction and expansion of IL-17-producing CD4 T cells during mycobacterial infection. J Immunol. 2006 Aug 1;177(3):1416-20. (RSG ELISA kit, TC supernatant, PubMed]

Kidoya H, Umemura M, Kawabe T, Matsuzaki G, Yahagi A, Imamura R, Suda T. Fas ligand induces cell-autonomous IL-23 production in dendritic cells, a mechanism for Fas ligand-induced IL-17 production. J Immunol. 2005 Dec 15;175(12):8024-31. (RSG ELISA kit, lung lavage fluid, PubMed)

Hegazi RA, Rao KN, Mayle A, Sepulveda AR, Otterbein LE, Plevy SE. Carbon monoxide ameliorates chronic murine colitis through a heme oxygenase 1–dependent pathway. J Exp Med. 2005 Dec 19;202(12):1703-13. [Mouse IL-23 ELISA]

Fichtner-Feigl S, Fuss IJ, Preiss JC, Strober W, Kitani A. Treatment of murine Th1- and Th2-mediated inflammatory bowel disease with NF-kappa B decoy oligonucleotides. J Clin Invest. 2005 Nov;115(11):3057-71. [Mouse IL-23 ELISA]

Zakharova M, Ziegler HK. Paradoxical Anti-Inflammatory Actions of TNF : Inhibition of IL-12 and IL-23 via TNF Receptor 1 in Macrophages and Dendritic Cells. J Immunol. 2005 Oct 15;175(8):5024-33. [Mouse IL-23 ELISA]

Happel KI, Dubin PJ, Zheng M, Ghilardi N, Lockhart C, Quinton LJ, Odden AR, Shellito JE, Bagby GJ, Nelson S, Kolls JK. Divergent roles of IL-23 and IL-12 in host defense against Klebsiella pneumoniae. J Exp Med. 2005 Sep 19;202(6):761-9. [Mouse IL-23 ELISA]

Kuwashima N, Nishimura F, Eguchi J, Sato H, Hatano M, Tsugawa T, Sakaida T, Dusak JE, Fellows-Mayle WK, Papworth GD, Watkins SC, Gambotto A, Pollack IF, Storkus WJ, Okada H. Delivery of dendritic cells engineered to secrete IFN-alpha into central nervous system tumors enhances the efficacy of peripheral tumor cell vaccines: dependence on apoptotic pathways. J Immunol. 2005 Aug 15;175(4):2730-40. [Mouse IL-23 ELISA]

Protocol

To view protocol, please download the TDS by clicking the PDF button above.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.