Analyte Specific Reagent (ASR): Analytical and performance characteristics are not established.
Fluorescence profiles of normal human peripheral blood monocytes unstained (blue histogram) or stained with PE anti-human CD38 (purple histogram).
Contents: CD38 PE Catalog Number: 8012-0388 Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Clone: HB7 Concentration: (test*): 5µl (0.06 µg) Host/Isotype: Mouse IgG1, k HLDA Workshop: III Storage Conditions: Store at 2-8°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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8012-0388-025
25 tests
8012-0388-120
120 tests
Test Sizes: *a test is defined as the amount that will stain 1x106 cells in 100 μL
The HB7 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is an ectoenzyme which catalyses NAD into nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR), both of which are secondary messengers. Expression of CD38 is bimodal during B cell development, modulating from high in immature cells to low in intermediate ones and back to high on mature B cells. Additionally CD38 is found in a variety of tissues and other hematopoietic cells (e.g. T cells, NK cells and monocytes). The CD34+CD38- population of hematopoietic stems cells is thought to define the most pluripotent cells (e.g. blast colony forming cells). In addition to surface expression, CD38 has recently been found in the nucleus where it may play a role in monitoring calcium levels.
References
Orciani M, Trubiani O, Guarnieri S, Ferrero E, Di Primio R. CD38 is constitutively expressed in the nucleus of human hematopoietic cells. J Cell Biochem. 2008 Oct 15;105(3):905-12.
Terstappen LW, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. Blood. 1991 Mar 15;77(6):1218-27.
Jackson DG, Bell JI. Isolation of a cDNA encoding the human CD38 (T10) molecule, a cell surface glycoprotein with an unusual discontinuous pattern of expression during lymphocyte differentiation. J Immunol. 1990 Apr 1;144(7):2811-5.
Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD38. In:
Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens.
New York, NY: Oxford University Press; 1989:86.
Pezzutto A, Behm F, Callard RE, et al. Flow cytometry analysis of the B-cell blind panel: joint report. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens.
New York, NY: Oxford University Press; 1989:165-174.
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