Contents: IkBalpha (C21) Blocking Peptide
Catalog Number: 66-P763
Formulation: Each vial contains 100 μg of peptide in 0.5 ml PBS with 0.1% NaN3 and 100 μg BSA.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
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Description
This is a peptide mapping to the carboxy terminal domain of human IkB-alpha. The NFkB family of transcription factors is sequestered in the cytoplasm by IkB inhibitory proteins (1&2).IkB-alpha and IkB-beta are the two members of this family most often cited for their regulatory function of NFkB directed gene expression. The interaction of NFkB dimers with IkB proteins keeps the complex in the cytoplasm, but upon cellular stimulation the IkB proteins are phosphorylated and subsequently degraded (1&3). The free NFkB can now enter the nucleus and induce gene expression of proteins critical for cellular proliferation, differentation, or apoptosis (1-4).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This blocking peptide has been reported for use in competition studies.
References
1. Schmitz ML, Baeuerle PA. 1995. Multi-step activation of NF-kappa B/Rel transcription factors. Immunobiology 193(2-4):116-127.
2. Tran K, Merika M, Thanos D. 1997. Distinct functional properties of IkappaB alpha and IkappaB beta. Mol Cell Biol 17(9):5386-5399.
3. Tanaka K, Kawakami T, Tateishi K, Yashiroda H, Chiba T. 2001. Control of IkappaBalpha proteolysis by the ubiquitin-proteasome pathway. Biochimie 83(3-4):351-356.
4. Chen CG, Malliaros J, Katerelos M, d?Apice AJ, Pearse MJ. Inhibition of NF-kappaB activation by a dominant-negative mutant of IkappaBalpha. Mol. Immunology 33(1):57-61.
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