Product Information

Contents: HIS tag (H20) Blocking Peptide
Catalog Number: 66-P757
Sizes: 50 ug
Formulation: Each vial contains 100 μg of peptide in 0.5 ml PBS with 0.1% NaN₃ and 100 μg BSA.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
66-P757	Blocking Peptides for Anti-polyhistidine Tag polyclonal	N/A	N/A	WB

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Description

This is a peptide containing the 6x histidine tag. The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This blocking peptide has been reported for use in competition studies.

Related Products

Cat. 14-6757 Purified Anti-polyhistidine Tag polyclonal (clone Polyclonal)