Product Information

Contents: AKT (C20) Blocking Peptide
Catalog Number: 66-P753
Sizes: 50 ug
Formulation: Each vial contains 100 μg of peptide in 0.5 ml PBS with 0.1% NaN₃ and 100 μg BSA.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
66-P753	Blocking Peptides for anti-mouse/human/rat AKT polyclonal	N/A	N/A	WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This is a synthetic peptide mapping to the carboxy terminal domain of human Akt1. Akt1, also known as Protein kinase B, is a serine/threonine kinase that is at the center of many signal transduction pathways. Akt1 regulates glucose metabolism, transcription, apoptosis, cell proliferation, angiogenesis, and cell motility (1). The activation of Akt1 occurs downstream of PI-3K, which upon activation by an extracellular molecule, generates PIP (3) (2). PIP (3) is a lipid second messenger that promotes the translocation of Akt1 to the plasma membrane via PH (pleckstrin-homology) domain binding (2-4). At the plasma membrane Akt1 is phosphorylated and activated by PDK-1 at its catalytic site on residue Thr308 (1). Residue Ser473 is also phosphorylated at this time but the kinase has not yet been identified (1). In this activated form Akt1 phosphorylates proteins that contain the motif, R-x-R-x-x-S/T-F/L, at the plasma membrane, in the cytosol, and in the nucleus (1). In addition to its kinase activity, Akt1 forms complexes with other proteins that are not substrates. These other proteins act as modulators of Akt1 activity (1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This blocking peptide has been reported for use in competition studies.

References

1. Brazil DP, Park J, Hemmings BA. 2002. PKB binding proteins. Getting in on the Akt. Cell 111 (3):293-303.
2. Nicholson KM, Anderson NG. 2002. The protein kinase B/Akt signalling pathway in human malignancy. Cell Signal 14(5): 381-395.
3. Marte BM, Downward J. 1997. PKB/Akt: connecting phosphoinositide 3-kinase to cell survival and beyond. Trends Biochem Sci 22(9): 355-358.
4. Coffer PJ, Jin J, Woodgett JR. 1998. Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem J. 335(Pt1): 1-13.

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