Contents: PUMA (I17) Blocking Peptide
Catalog Number: 66-P740
Formulation: Each vial contains 100 μg of peptide in 0.5 ml PBS with 0.1% NaN3 and 100 μg BSA.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
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Description
This is a peptide mapping to an internal domain of human PUMA. The Bcl-2 family proteins can function to either suppress or promote cell death and are characterized by the presence of up to four conserved amino acid motifs, termed Bcl-2 homology (BH) domains (1). The BH3 function has been further revealed by an emerging group of BH3-only proteins, that includes Bik, Bad, Bid, Bim, Noxa, and the newest member PUMA or bbc3 (1&2). The PUMA gene is a target of p53 transcriptional activation and encodes two BH3 domain-containing proteins (PUMA-alpha and PUMA-beta) (3&4). The activities of PUMA-alpha and PUMA-beta are similar; they bind to Bcl-2, localize to the mitochondria to induce cytochrome c release, and activate extremely rapid apoptosis (3&4).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This blocking peptide has been reported for use in competition studies.
References
1. Han J., Flemington C., Houghton A.B., Gu Z., Zambetti G.P., Lutz R.J., Zhu L., Chittenden T. 2001. Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals. PNAS 98(20):11318-11323.
2. Schuler M., Green D.R. 2001. Mechanisms of p53-dependent apoptosis. Biochem Soc Trans 29(Pt6):684-688.
3. Nakano K., Vousden K.H. 2001. PUMA, a novel proapoptotic gene, is induced by p53. Mol Cell 7(3):683-694.
4. Yu J., Zhang L., Hwang P.M., Kinzler K.W., Vogelstein B. 2001. PUMA induces the rapid apoptosis of colorectal cancer cells. Mol Cell 7(3):673-682.
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