Product Information

Contents: p53 (I20) Blocking Peptide
Catalog Number: 66-P736
Sizes: 50 ug
Formulation: Each vial contains 100 μg of peptide in 0.5 ml PBS with 0.1% NaN₃ and 100 μg BSA.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
66-P736	Blocking Peptides for anti-human p53 polyclonal	N/A	N/A	WB

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Description

This is a peptide mapping to an internal domain of Human p53. The p53 tumor suppressor gene encodes a transcription factor that contributes to several cellular activities that include apoptosis, transient growth arrest, and sustained growth arrest or senescence (1). Mutations within the p53 gene are found in about half of all human cancers (2). In cells that are functioning normally the MDM2 protein binds to p53 and maintains p53 at low levels by increasing its susceptibility to proteolysis by the 26S proteosome (3). A cell that undergoes stress loses the ability of MDM2 to bind to p53 and as a result p53 levels increase which then leads to cell cycle arrest or apoptosis (3&4). p53 induced cell cycle arrest or apoptosis can be achieved through transcriptional regulation of several genes including the cell cycle inhibitor p21, DNA repair gene GADD45, and the apoptotic inducer Bax (5). Besides MDM2 inactivation, p53 can also be functionally inactivated by mutation or binding to DNA tumor virus encoded proteins, such as SV40 large T antigen, Adenovirus E1B and papilloma virus E6 proteins (6).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This blocking peptide has been reported for use in competition studies.

References

1. Bargonetti J, Manfredi JJ. 2002. Multiple Roles of the tumor suppressor p53. Curr Opin Oncol 14(1):86-91
2. Vousden KH. 2002. Activation of the p53 tumor suppressor protein. Biochim Biophys Acta 1602(1):47-59
3. Momand J, Wu HH, Dasgupta G. 2000. MDM2?master regulator of the p53 tumor suppressor protein. Gene 242(1-2):15-29
4. Alarcon-Vargas D, Ronai Z. 2002. p53-MDM2?the affair that never ends. Carcinogenesis 23(4):541-547
5. Gong B, Almasan A. 1999. Differential upregulation of p53-responsive genes by genotoxic stress in hematopoietic cells containing wild-type and mutant p53. Gene Expr 8(4):197-206
6. Kaelin WG Jr. 1999. The emerging p53 gene family. J Natl Cancer Inst 91(7):594-598.

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