Contents: c-Fos (A14) Blocking Peptide Catalog Number: 66-P723 Formulation: Each vial contains 100 μg of peptide in 0.5 ml PBS with 0.1% NaN3 and 100 μg BSA. Storage Conditions: Store at 4°C. DO NOT FREEZE.
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66-P723-81
50 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
This is a peptide mapping to the amino terminus of human c-Fos. The products of the proto-oncogenes c-Jun (c-Jun was previously known as the Fos-binding protein p39) and c-Fos are known to form a complex in the nucleus (2, 6). AP-1 (activating protein-1) is a collective term referring to these dimeric transcription factors composed of Jun, Fos or ATF (activating transcription factor) subunits that bind to a common DNA site, the AP-1-binding site (1). There is evidence that AP-1 proteins, mostly those that belong to the Jun group, control cell life and death through their ability to regulate the expression and function of cell cycle regulators such as Cyclin D1, p53, p21 (cip1/waf1), p19 (ARF) and p16 (3). The Fos and Jun proto-oncogenes expression is induced transiently by a great variety of extracellular stimuli associated with mitogenesis, differentiation processes or depolarization of neurons (5). Studies have established a unique role for Fos in determining the differentiation and activity of progenitors of the osteoclast lineage (4).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This blocking peptide has been reported for use in competition studies.
References
1. Karin M, Liu Zg, Zandi E. AP-1 function and regulation.Curr Opin Cell Biol. 1997 Apr;9(2):240-6.
2. Bos TJ, Rauscher FJ 3rd, Curran T, Vogt PK. The carboxy terminus of the viral Jun oncoprotein is required for complex formation with the cellular Fos protein. Oncogene. 1989 Feb;4(2):123-6.
3. Shaulian E, Karin M. AP-1 in cell proliferation and survival. Oncogene. 2001 Apr 30;20(19):2390-400.
4. Liebermann DA, Gregory B, Hoffman B. AP-1 (Fos/Jun) transcription factors in hematopoietic differentiation and apoptosis. Int J Oncol. 1998 Mar;12(3):685-700
5. Curran T. Fos and Jun: oncogenic transcription factors.Tohoku J Exp Med. 1992 Oct;168(2):169-74.
6. Rauscher FJ 3rd, Voulalas PJ, Franza BR Jr, Curran T. Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro. Genes Dev. 1988 Dec;2(12B):1687-99.
