Product Information

Contents: c-Src (A16) Blocking Peptide
Catalog Number: 66-P721
Sizes: 50 ug
Formulation: Each vial contains 100 μg of peptide in 0.5 ml PBS with 0.1% NaN₃ and 100 μg BSA.
Storage Conditions: Store at 4°C.
DO NOT FREEZE.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
66-P721	Blocking Peptides for anti-mouse/human/rat cSrc polyclonal	N/A	N/A	WB

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Description

This is a peptide mapping to the amino terminus of human c-Src p60. The Src gene family is represented by at least eight different protein tyrosine kinases belonging to the non-receptor tyrosine kinases (1,2). These protein tyrosine kinases are important regulators of many cellular processes, including cytoskeletal organization, cell-cell contact, DNA synthesis, and cellular proliferation (1-3). Members of this group of proteins include c-Src, c-Yes, Fyn, Lck, Lyn, Hck, Blk and c-Fgr. The proto-oncogene c-Src is the prototype member of this gene family and is expressed in a broad range of tissues and cells (4,5). Elevated c-Src tyrosine kinase activity has been found in many types of human cancers, most notably in breast carcinomas (6,7). The activity of c-Src in these human cancers has been attributed to increases c-Src expression, elevated c-Src specific activity and activating mutations in c-Src (6,7).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This blocking peptide has been reported for use in competition studies.

References

1. Oda H, Kumar S, Howley PM. 1999. Regulation of the Src family tyrosine kinase Blk through E6AP-mediated ubiquitination. Proc Natl Acad Sci USA 96(17):9557-9562.
2. Biscardi JS, Ishizawar RC, Silva CM, Parsons SJ. 2000. Tyrosine kinase signalling in breast cancer: epidermal growth factor receptor and c-Src interaction in breast cancer. Breast Cancer Res 2(3):203-210.
3. Ingraham CA, Cox ME, Ward DC, Fults DW, Maness PF. 1989. c-Src and other proto-oncogenes implicated in neuronal differentiation. Mol Chem Neuropathol 10(1):1-14.
4. Wiestler OD, Walter G. 1988. Developmental expression of two forms of pp60c-src in mouse brain. Mol Cell Biol 8(1):502-504.
5. Yang XM, Martinez R, Le Beau J, Wiestler O, Walter G. Evolutionary expression of the neuronal form of the src protein. Proc Natl Acad Sci USA 86(12):4751-4755.
6. Bjorge JD, Pang A, Fujita DJ. 2000. Identification of protein-tyrosine phosphatase 1B as the major tyrosine phosphatase activity capable of dephosphorylating and activating c-Src in several human breast cancer cell lines. J Biol Chem 275(52):41439-41446.
7. Hung W, Elliot B. 2001. Co-operative effect of c-Src tyrosine kinase and Stat3 in activation of hepatocyte growth factor expression in mammary carcinoma cells. J Biol Chem 276(15):12395-12403.

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