Catalog Number: 66-P222
Storage Conditions: It is supplied as 200 µg/ml, 50 µg/vial, in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM
NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20°C, stable for one year.
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Description
This peptide is composed of amino acids 233 to 248 (CDFQTKFALSLSPGAHD) of human MyD88. MyD88, a myeloid differentiation primary response gene, is expressed in a variety of tissues and functions as an adapter molecule in the IL-1 signaling pathway involved in the inflammatory responses induced by cytokines and LPS. MyD88 associates with and recruits IRAK to the IL-1 receptor. Dominant negative mutants of MyD88 attenuate IL-1R-mediated NF-κB activation. MyD88 also functions as a regulator molecule for IL-18 receptor and human Toll receptor family. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to LPS.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
The peptide is used for blocking the activity of anti-MyD88. Incubating the peptide with equal volume of antibody for 30 min at 37°C usually completely blocks the antibody activity in Western blotting.
References
Bonnert TP, Garka KE, Parnet P, Sonoda G, Testa JR, and Sims JE. 1997. The cloning and characterization of human MyD88: a member of an IL-1 receptor related family. FEBS Lett. 402:81-4.
Human Cellular Antigens Chart