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Contents: Affinity Purified anti-pro-Caspase 3 Catalog Number: 60-6673 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles Clone: 31A893 Isotype: Mouse IgG1 Prices for This Product* Cat. No. Size Price Qty Action 60-6673-81 50 ug $110 60-6673-82 100 ug $170 *International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
60-6673	anti-pro-Caspase 3	N/A	N/A	WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The 31A893 antibody reacts with pro-caspase 3. The gene for Caspase-3, also known as Yama, CPP32, and apopain, codes for a 32 kDa protein. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17 kDa and a small subunit of 12 kDa.
The 31A893 antibody was raised against full-length recombinant human caspase-3. The antibody was selected with staurosporine-induced apoptotic HeLa cell lysate.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The 31A893 antibody has been reported for use in immunoblotting (WB).

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Applications Tested

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The 31A893 antibody is useful for detecting pro-Caspase-3 in cells by Western blot analysis, and can be used at 2 μg/ml dilution. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

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References

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Fernandes-Almnemri T, et al. J Biol Chem 269: 30761-764 (1994).
Tewari M, et al. Cell 81: 801-809 (1995).
Shi L, et al. Proc Natl Acad Sci USA 93: 11002-007 (1996).
Villa P, et al. TIBS 22: 389-392 (1997).

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