Contents: anti-mouse/human Caspase-3 (active, pro-Caspase-3)
Catalog Number: 60-6663
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: This product is supplied as Protein G purified IgG fraction in PBS containing 0.02% sodium azide. Store at 4°C, stable for 6 months. For long-term storage, store at –20°C.
Clone: 31A1067
Isotype: Mouse IgG1
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Description
The 31A1067 antibody reacts with mouse and human active and pro-caspase 3. The gene for caspase-3, also known as Yama, CPP32, and apopain, codes for a 32 kDa protein. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17 kDa and a small subunit of 12 kDa. The 31A1067l antibody was generated against recombinant protein of full-length human caspase-3. The antibody was selected with staurosporine-induced apoptotic HeLa cell lysate.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 31A1067 antibody has been reported for use in immunoblotting (WB).
Applications Tested
The 31A1067 antibody is useful for detecting pro-Caspase-3 and Caspase-3 activation in cells by Western blot analysis at a recommended dilution of 2 μg/ml. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.
References
Fernandes-Almnemri T, et al. J Biol Chem 269: 30761-764 (1994).
Tewari M, et al. Cell 81: 801-809 (1995).
Shi L, et al. Proc Natl Acad Sci USA 93: 11002-007 (1996).
Villa P, et al. TIBS 22: 389-392 (1997).
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