Product Information

Contents: Affinity Purified anti-human RANK CD265, Receptor Activator of NF-κB)
Catalog Number: 60-6618
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C. Avoid repeated freeze/thaw cycles.
Clone: 9A725
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
60-6618	anti-human RANK (CD265, Receptor Activator of NF-kB)	N/A	N/A	WB

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Description

The 9A725 antibody reacts with human RANK. Receptor activator of NF-κB (RANK) is a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-X_L suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-κB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-κB, whereas JNK activation is mediated through binding of RANK to TRAF2.
A GST-fusion protein containing amino acid residues 326-616 of human RANK was used as immunogen. This antibody recognizes an epitope located between amino acid residues 330-427 of human RANK.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 9A725 antibody has been reported for use in immunoblotting (WB).

Applications Tested

The 9A725 antibody can be used for detection of RANK expression by Western blot at 1μg/ml. Activated T cells can be used as positive control and a protein of approximate molecular weight of 97 kDa should be detected. It is recommended that the reagent be carefully titrated for optimal performance in the assay of interest.

References

Anderson DM, et al. Nature 390: 175-179 (1997).
Wong, BR, et al. J Exp Med 186: 2075-2080.
Darnay, BG, et al. J Biol Chem 273: 20551-555 (1998).
Darnay, BG, et al. J Biol Chem 274: 7724-7731 (1999).