Product Information

Contents: Pacific Blue® anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)
Catalog Number: 57-7321
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MP6-XT22
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were fixed and permeabilized and subsequently stained with 0.125 ug Pacific Blue anti-mouse TNF-a (MP6-XT22) (right) or staining buffer (autofluoresence) (left).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7321	FITC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	518	IC Flow
12-7321	PE anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	575	IC Flow
14-7321	Affinity Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IC Flow IH/F
16-7321	Functional Grade* Purified anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	NU
17-7321	APC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	660	IC Flow
19-7321	Cy5 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	670	IC Flow
51-7321	Alexa Fluor® 647 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	668	IC Flow
53-7321	Alexa Fluor® 488 anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	519	IC Flow
57-7321	Pacific Blue® anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP6-XT22 antibody reacts with mouse tumor necrosis factor-alpha (TNF-α), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane-bound form.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MP6-XT22 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This MP6-XT22 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hunter, C. A., M. J. Litton, et al. (1994). "Immunocytochemical detection of cytokines in the lymph nodes and brains of mice resistant or susceptible to toxoplasmic encephalitis." J Infect Dis 170(4): 939-45.
Litton, M. J., B. Sander, et al. (1994). "Early expression of cytokines in lymph nodes after treatment in vivo with Staphylococcus enterotoxin B." J Immunol Methods 175(1): 47-58.
Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

Related Products

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Cat. 12-7423    Phycoerythrin (PE) anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 14-7423    Affinity Purified anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 16-7423    Functional Grade Purified anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 25-7423    Phycoerythrin-Cy7 (PE-Cy7) anti-mouse/rat TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone TN3-19)
Cat. 36-7423    In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade Biotin anti-mouse TNFa (Tumor Necrosis Factor-alpha, TNF-a) (clone TN3-19)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.