Product Information

Contents: Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g)
Catalog Number: 57-7319
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4S.B3
Isotype: Mouse IgG1, κ

Intracellular staining of 6 hour PMA/ionmycin-stimulated normal human peripheral blood cells with PE anti-human CD4 (RPA-T4) (cat. 12-0049) and staining buffer (autofluorescence) (left) or Pacific Blue® anti-human IFNγ (4S.B3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7319	Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	IC Flow
13-7319	Biotin anti-human IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA det ELISPOT
11-7319	FITC anti-human IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7319	PE anti-human IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
17-7319	APC anti-human IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
25-7319	Phycoerythrin-Cy7 (PE-Cy7) anti-human IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
51-7319	Alexa Fluor® 647 anti-human IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7319	Alexa Fluor® 488 anti-human IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7319	Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
45-7319	PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 4S.B3 monoclonal antibody reacts with interferon-γ (IFN-γ). The 4S.B3 antibody is a neutralizing antibody. Human IFN-γ is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This 4S.B3 antibody has been pre-titrated and tested . This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon." J Interferon Res 4(4): 619-25.

Related Products

Cat. 88-7916 Human IFNg (Interferon-gamma, IFN-g) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.