Contents: Pacific BlueŽ anti-human IFNg (Interferon-gamma, IFN-g)
Catalog Number: 57-7319
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 4S.B3
Isotype: Mouse IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
 
Intracellular staining of 6 hour PMA/ionmycin-stimulated normal human peripheral blood cells with PE anti-human CD4 (RPA-T4) (cat. 12-0049) and staining buffer (autofluorescence) (left) or Pacific BlueŽ anti-human IFNγ (4S.B3) (right). Cells in the lymphocyte gate were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-7319 |
FITC anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
518 |
IC Flow
|
| 12-7319 |
PE anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
575 |
IC Flow
|
| 13-7319 |
Biotin anti-human IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
ELISA det
ELISPOT
|
| 14-7319 |
Affinity Purified anti-human IFNg (Interferon-gamma, IFN-g) |
N/A |
N/A |
IC Flow
|
| 17-7319 |
APC anti-human IFNg (Interferon-gamma, IFN-g) |
633 |
660 |
IC Flow
|
| 25-7319 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
760 |
IC Flow
|
| 45-7319 |
PerCP-Cy5.5 anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
690 |
IC Flow
|
| 51-7319 |
Alexa Fluor® 647 anti-human IFNg (Interferon-gamma, IFN-g) |
633 |
668 |
IC Flow
|
| 53-7319 |
Alexa Fluor® 488 anti-human IFNg (Interferon-gamma, IFN-g) |
488 |
519 |
IC Flow
|
| 57-7319 |
Pacific Blue® anti-human IFNg (Interferon-gamma, IFN-g) |
402 |
421 |
IC Flow
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 4S.B3 monoclonal antibody reacts with interferon-γ (IFN-γ). The 4S.B3 antibody is a neutralizing antibody. Human IFN-γ is a 17 kDa factor produced by activated T and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 4S.B3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
The Pacific BlueŽ emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This 4S.B3 antibody has been pre-titrated and tested . This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.
References
Meager, A., S. Parti, et al. (1984). "Detection of hybridomas secreting monoclonal antibodies to human gamma interferon using a rapid screening technique and specificity of certain monoclonal antibodies to gamma interferon."
J Interferon Res 4(4): 619-25.
Related Products
Cat.
88-7916 Human IFNg (Interferon-gamma, IFN-g) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
