Product Information

Contents: Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g)
Catalog Number: 57-7311
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: XMG1.2
Isotype: Rat IgG1, κ

Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. Cells were then stained intracellularly with staining buffer (autofluorescence) (left) or 0.25 μg of Pacific Blue® anti-mouse IFNg (XMG1.2) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7311	FITC anti-mouse IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7311	PE anti-mouse IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
13-7311	Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA
14-7311	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap
16-7311	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
17-7311	APC anti-mouse IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
19-7311	Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	670	IC Flow
25-7311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
45-7311	PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow
51-7311	Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7311	Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7311	Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The XMG1.2 antibody reacts with mouse interferon-γ. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-γ is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The XMG1.2 antibody has been reported for use in capture or detection of mouse IFN-γ by sandwich ELISA, for intracellular staining and flow cytometric analysis, for immunoblotting (WB), and for neutralization of IFN-γ bioactivity (use Functional Grade purified cat. 16-7311).

Applications Tested

The Pacific Blue® conjugated XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis on activated mouse splenocytes. The optimal concentration is typically 0.25 µg per million cells in 100 µL staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

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Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.