Product Information

Contents: Pacific Blue® anti-human IL-12 (Interleukin-12) p40/70
Catalog Number: 57-7129
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C8.6
Isotype: Mouse IgG1, κ

Human PBMCs were primed with IFNγ for 2 hrs, then activated with LPS for 22 hrs in the presence of monensin. Cells were then intracellularly stained with staining buffer (autofluorescence) (left) or Pacific Blue® anti-human IL-12 p40/70 (C8.6) (right). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7129	Affinity Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	IC Flow NU WB
16-7129	Functional Grade* Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	NU
13-7129	Biotin anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	ELISA det
12-7129	PE anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	488	575	IC Flow
51-7129	Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	633	668	IC Flow
53-7129	Alexa Fluor® 488 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	488	519	IC Flow
57-7129	Pacific Blue® anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C8.6 antibody has been reported for use in ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested

This C8.6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Cho, D., et al. 1996. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 172: 29-34.
Aragane, Y, et al. 1994. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153: 5366-72.

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Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.