57-7129

Contents: Pacific Blue® anti-human IL-12 (Interleukin-12) p40/70
Catalog Number: 57-7129
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C8.6
Isotype: Mouse IgG1, κ

Product Options

Price

Cat. No.

Size

To view relevant pricing,
please select your country
and resume browsing.

Human PBMCs were primed with IFNγ for 2 hrs, then activated with LPS for 22 hrs in the presence of monensin. Cells were then intracellularly stained with staining buffer (autofluorescence) (left) or Pacific Blue® anti-human IL-12 p40/70 (C8.6) (right). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7129	Affinity Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	IC Flow NU WB
16-7129	Functional Grade* Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	NU
13-7129	Biotin anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	N/A	N/A	ELISA det
12-7129	PE anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	488	575	IC Flow
51-7129	Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	633	668	IC Flow
53-7129	Alexa Fluor® 488 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70	488	519	IC Flow
48-7129	eFluor^TM 450 anti-human IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40/70 (Pacific Blue® replacement)	405	450	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C8.6 antibody has been reported for use in ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested

This C8.6 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Cho, D., et al. 1996. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 172: 29-34.
Aragane, Y, et al. 1994. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153: 5366-72.

Related Products

Cat. 00-4505  eBioscience Monensin Solution
Cat. 14-7238  Affinity Purified anti-human IL-23 (Interleukin-23, IL23) p19 (clone eBio473P19)
Cat. 14-8129  Recombinant Human IL-12 (Interleukin-12, IL12) p70
Cat. 00-8222  eBioscience IC Fixation Buffer
Cat. 34-8239  Carrier-Free Recombinant Human IL-23 (Interleukin-23, IL23)
Cat. 00-8333  eBioscience Permeabilization Buffer(10X)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.