Contents: Pacific Blue® anti-human IL-10 (Interleukin-10, IL10)
Catalog Number: 57-7108
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: JES3-9D7
Isotype: Rat IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of unstimulated (left) and PMA/ionomycin stimulated (right) CD4 sorted PBMCs cultured under Th2 polarizing conditions with surface PE anti-human CD3 (UCHT1) (cat. 12-0038) and intracellular Pacific Blue® anti-human IL-10 (JES3-9D7). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 12-7108 |
PE anti-human IL-10 (Interleukin-10, IL10) |
488 |
575 |
IC Flow
|
| 14-7108 |
Affinity Purified anti-human IL-10 (Interleukin-10, IL10) |
N/A |
N/A |
ELISA cap
ELISPOT cap
IHC(Paraffin)
|
| 16-7108 |
Functional Grade* Purified anti-human IL-10 (Interleukin-10, IL10) |
N/A |
N/A |
ELISPOT cap
NU
|
| 51-7108 |
Alexa Fluor® 647 anti-human IL-10 (Interleukin-10, IL10) |
633 |
668 |
IC Flow
|
| 53-7108 |
Alexa Fluor® 488 anti-human IL-10 (Interleukin-10, IL10) |
488 |
519 |
IC Flow
|
| 57-7108 |
Pacific Blue® anti-human IL-10 (Interleukin-10, IL10) |
402 |
421 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis. This can be used at 20 μl (1 μg) per million cells in a 100 μl total staining volume.
References
Abrams, J. S., M. G. Roncarolo, et al. 1992. Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev 127: 5-24.
Chandler SW, Rassekh CH, et al. 2002. Immunohistochemical localization of interleukin-10 in human oral and pharyngeal carcinomas. Laryngoscope. 112(5):808-15. (IHC paraffin,
PubMed)
Sendide K, Deghmane AE, et al. 2005. Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S. J Immunol. 175(8):5324-32. (FA,
PubMed)
Related Products
Cat.
57-4301 Pacific Blue® Rat IgG1 Isotype Control
Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
