Product Information

Contents: Pacific Blue® anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)
Catalog Number: 57-5941
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: PK136
Isotype: Mouse IgG2a, κ

Staining of C57Bl/6 splenocytes 0.25 μg of Pacific Blue® Mouse IgG2a, K Iso Cntrl (cat. 57-4724) (left) or 0.25 μg of Pacific Blue® anti-mouse NK-1.1 (PK136) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5941	FITC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	488	518	FC
12-5941	PE anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	488	575	FC
13-5941	Biotin anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	N/A	N/A	FC
14-5941	Affinity Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	N/A	N/A	FC IP
16-5941	Functional Grade* Purified anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	N/A	N/A	FC
17-5941	APC anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	633	660	FC
25-5941	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	488	760	FC
45-5941	PerCP-Cy5.5 anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	488	690	FC
57-5941	Pacific Blue® anti-mouse NK-1.1 (NK1.1, NKR-P1C, Ly-55)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5⁺PK136^- and DX5^-PK136⁺ cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This PK136 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This PK136 antibody has been tested by flow cytometric analysis of C57BL/6 splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Koo, G. C. and J. R. Peppard (1984). "Establishment of monoclonal anti-Nk-1.1 antibody." Hybridoma 3(3): 301-3.

Kitaichi N, Kotake S, Morohashi T, Onoe K, Ohno S, Taylor AW. Diminution of experimental autoimmune uveoretinitis (EAU) in mice depleted of NK cells. J Leukoc Biol. 2002 Dec;72(6):1117-21. (PK136, in vivo depletion, PubMed)

Related Products

Cat. 57-4724 Pacific Blue® Mouse IgG2a, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.