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Refer to NEW Anti-Mouse CD309 (FLK1) eFluorŽ 450 (Pacific BlueŽ replacement)

Also known as: Flk-1, VEGF-R2, VEGFR2, Ly-73, Ly73, KDR

RUO: For Research Use Only
mouse Flk-1 antibody, Pacific BlueŽ conjugatemouse Flk-1 antibody, Pacific BlueŽ conjugateStaining of bEND.3 cell line with 0.25 ĩg of Pacific BlueŽ Rat IgG2a κ Isotype Control(cat. 57-4321) (left histogram) or 0.25 ĩg of Pacific BlueŽ Anti-Mouse Flk-1 (Avas12a1) (right histogram). Total viable cells were used for analysis.
Contents: Refer to NEW Anti-Mouse CD309 (FLK1) eFluorŽ 450 (Pacific BlueŽ replacement)
Catalog Number: 57-5821
Concentration: 0.2 mg/ml
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: Avas12a1
Host/Isotype: Rat IgG2a, κ
 
Product Options
Cat. No. Size Price Add Qty to Cart
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
57-4321 Refer to NEW Rat IgG2a K Isotype Control eFluorŽ 450 (Pacific BlueŽ replacement)
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
12-5821 Anti-Mouse CD309 (FLK1) PE 488 575 FC RUO
13-5821 Anti-Mouse CD309 (FLK1) Biotin N/A N/A FC RUO
14-5821 Anti-Mouse CD309 (FLK1) Purified N/A N/A FC IHC WB RUO
17-5821 Anti-Mouse CD309 (FLK1) APC 633 660 FC RUO
56-5821 Anti-Mouse CD309 (FLK1) Alexa Fluor® 700 633 723 FC RUO
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŽ 647 conjugated products with eFluorŽ 660 conjugated products.
 
Description
The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This Avas12a1 antibody has been reported for use in flow cytometric analysis.

The Pacific BlueŽ emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This Avas12a1 antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 ĩL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References

Kataoka, H., N. Takakura, et al. (1997)."Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells." Dev Growth Differ 39(6): 729-40.
Legal
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.