Product Information

Contents: Pacific Blue® anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra)
Catalog Number: 57-1271
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A7R34
Isotype: Rat IgG2a, κ

Staining of mouse splenocytes with FITC anti-mouse/human CD45R(B220) (cat.11-0452) and Pacific Blue rat IgG2a isotype control (left) or 0.25 µg Pacific Blue anti-mouse CD127 (A7R34) (right).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-1271	FITC anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	518	FC
12-1271	PE anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	575	FC
13-1271	Biotin anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	N/A	N/A	FC
14-1271	Affinity Purified anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	N/A	N/A	FA FC IH/F IP
15-1271	PE-Cy5 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	670	FC
16-1271	Functional Grade* Purified anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	N/A	N/A	FA FC
17-1271	APC anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	633	660	FC
25-1271	PE-Cy7 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	760	FC
27-1271	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) (APC-Cy7 Equivalent)	633	779	FC
45-1271	PerCP-Cy5.5 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	690	FC
51-1271	Alexa Fluor® 647 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	633	668	FC
53-1271	Alexa Fluor® 488 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	488	519	FC
56-1271	Alexa Fluor® 700 anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra) (APC-Cy5.5 Equivalent)	633	723	FC
57-1271	Pacific Blue® anti-mouse CD127 (Interleukin-7 Receptor alpha, IL-7 Receptor alpha, IL-7Ra, IL7Ra)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity α subunit of the mouse IL-7 receptor. IL-7 receptor α chain is expressed by immature B cells in the bone marrow, double-negative (CD4^-/CD8^-), single-positive (CD4⁺ and CD8⁺), but not double-positive (CD4⁺/CD8⁺) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This A7R34 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This A7R34 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sudo, T., S. Nishikawa, et al. 1993. Expression and function of the interleukin 7 receptor in murine lymphocytes. Proc Natl Acad Sci U S A 90(19): 9125-9.
Ohana M, Okazaki K, Oshima C, et al. 2001. A critical role for IL-7R signaling in the development of Helicobacter felis-induced gastritis in mice. Gastroenterology. Aug;121(2):329-36.
Okuno Y, Iwasaki H, Huettner CS, Radomska HS, Gonzalez DA, Tenen DG, Akashi K. 2002. Differential regulation of the human and murine CD34 genes in hematopoietic stem cells. Proc Natl Acad Sci U S A. Apr 30;99(9):6246-51.

Related Products

Cat. 57-4321 Pacific Blue® Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.