mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb)

Contents: Pacific Blue® anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb)
Catalog Number: 57-1222
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: TM-b1 (TM-beta1)
Isotype: Rat IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
57-1222-80	25 ug	$140
57-1222-82	100 ug	$275

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1222	Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FC IP NU
16-1222	Functional Grade* Purified anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FA FC
13-1222	Biotin anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	N/A	N/A	FC
11-1222	Fluorescein isothiocyanate (FITC) anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	488	518	FC
12-1222	Phycoerythrin (PE) anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb, IL2Rb)	488	575	FC
57-1222	Pacific Blue® anti-mouse CD122 (Interleukin-2 Receptor beta, IL-2 Receptor beta, IL-2Rb)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TM-β1 monoclonal antibody reacts with mouse CD122, the 90-110 kDa interleukin-2 receptor β chain (IL-2Rβ). CD122 is expressed by NK cells and some T cells in the periphery, and is upregulated by activation. CD122 associates with CD25 and CD132 (common γ chain) to form the high affinity IL-2 receptor. High affinity binding of IL-2 to the IL-2 receptor is inhibited by the TM-β1 antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This TM-b1 (TM-beta1) antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This TM-b1 (TM-beta1) antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Takeuchi Y, Tanaka T, Hamamura K, Sugimoto T, Miyasaka M, Yagita H, Okumura K. 1992. Expression and role of interleukin-2 receptor beta chain on CD4-CD8- T cell receptor alpha beta+ cells. Eur J Immunol. 22(11):2929-35.

Tanaka T, Takeuchi Y, Shiohara T, Kitamura F, Nagasaka Y, Hamamura K, Yagita H, Miyasaka M. 1992. In utero treatment with monoclonal antibody to IL-2 receptor beta-chain completely abrogates development of Thy-1+ dendritic epidermal cells. Int Immunol. 4(4):487-91.

French JD, Roark CL, Born WK, O'brien RL. {gamma}{delta} T cell homeostasis is established in competition with {alpha}{beta} T cells and NK cells. Proc Natl Acad Sci U S A. 2005 Oct 11;102(41):14741-6. Epub 2005 Oct 3. (blocking, PubMed)

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Cat. 57-4031 Pacific Blue® Rat IgG2b Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.