human CD117 (c-Kit, cKit)

Contents: Pacific Blue® anti-human CD117 (c-Kit)
Catalog Number: 57-1179
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: YB5.B8
Isotype: Mouse IgG1, κ
HLDA No.: V C009

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
57-1179-71	25 tests	$150
57-1179-73	100 tests	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of TF-1 cells with 0.25 μg of Pacific Blue® Mouse IgG1, K Iso Cntrl (cat. 57-4714) (blue histogram) or Pacific Blue® anti-human CD117 (YB5.B8) (purple histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1179	PE anti-human CD117 (c-Kit)	488	575	FC
13-1179	Biotin anti-human CD117 (c-Kit)	N/A	N/A	FC
14-1179	Affinity Purified anti-human CD117 (c-Kit)	N/A	N/A	FC IH/F IP
17-1179	Allophycocyanin (APC) anti-human CD117 (c-Kit)	633	660	FC
57-1179	Pacific Blue® anti-human CD117 (c-Kit)	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit , Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This YB5.B8 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This YB5.B8 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Lerner NB, Nocka KH, Cole SR, Qiu FH, Strife A, Ashman LK, Besmer P. Monoclonal antibody YB5.B8 identifies the human c-kit protein product. Blood 1991 May 1;77(9):1876-83
Okayama Y, Hunt TC, Kassel O, Ashman LK, Church MK. Assessment of the anti-c-kit monoclonal antibody YB5.B8 in affinity magnetic enrichment of human lung mast cells.
J Immunol Methods 1994 Mar 10;169(2):153-61
Ashman LK, Buhring HJ, Aylett GW, Broudy VC, Muller C. Epitope mapping and functional studies with three monoclonal antibodies to the c-kit receptor tyrosine kinase, YB5.B8, 17F11, and SR-1. J Cell Physiol 1994 Mar;158(3):545-54

Related Products

Cat. 57-4714 Pacific Blue® Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.