Product Information

Contents: Pacific Blue® anti-mouse CD105 (Endoglin)
Catalog Number: 57-1051
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MJ7/18
Isotype: Rat IgG2a, κ

Staining of BEND3 cells with 0.5 μg of Pacific Blue® Rat IgG2a Iso Cntrl (cat. 57-4321) (blue histogram) or 0.5 μg of Pacific Blue® anti-mouse CD105 (MJ7/18)) (purple histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1051	Affinity Purified anti-mouse CD105 (Endoglin)	N/A	N/A	FC IH/F IP WB
13-1051	Biotin anti-mouse CD105 (Endoglin)	N/A	N/A	FC
12-1051	Phycoerythrin (PE) anti-mouse CD105 (Endoglin)	488	575	FC
57-1051	Pacific Blue® anti-mouse CD105 (Endoglin)	402	421	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MJ7/18 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This MJ7/18 antibody has been tested by flow cytometric analysis on bEND3 cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ge, A. Z. and E. C. Butcher. 1994. Cloning and expression of a cDNA encoding mouse endoglin, an endothelial cell TGF-beta ligand. Gene 138(1-2): 201-6.

Tokita D, Ohdan H, Onoe T, Hara H, Tanaka Y, Asahara T. Liver sinusoidal endothelial cells contribute to alloreactive T-cell tolerance induced by portal venous injection of donor splenocytes.Transpl Int. 2005 Feb;18(2):237-45.

Quintanilla M, Ramirez JR, et al. 2003. Expression of the TGF-beta coreceptor endoglin in epidermal keratinocytes and its dual role in multistage mouse skin carcinogenesis. Oncogene. 22(38):5976-85. (MJ7/18, IHC formalin-fixed and WB, PubMed)

Onoe T, Ohdan H, et al. 2005. Liver sinusoidal endothelial cells tolerize T cells across MHC barriers in mice. J Immunol. 175(1):139-46. (MJ7/18, IHC frozen, PubMed)

Korpanty G, Carbon JG, et al. 2007. Monitoring response to anticancer therapy by targeting microbubbles to tumor vasculature. Clin Cancer Res. 13(1):323-30. (MJ7/18, IHC frozen, PubMed)

Perez-Gomez E, Villa-Morales M, et al. 2007. A role for endoglin as a suppressor of malignancy during mouse skin carcinogenesis. Cancer Res. 67(21):10268-77. (MJ7/18, WB, PubMed)

Related Products

Cat. 57-4321 Pacific Blue® Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.