Product Information

Contents: Pacific Blue® anti-human CD95 (Fas/APO-1)
Catalog Number: 57-0959
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DX2
Isotype: Mouse IgG1, κ
HLDA No.: VI C64

Staining of normal human peripheral blood cells with PE anti-human CD19 (HIB19) (cat. 12-0199) and 0.5 μg of Pacific Blue® Mouse IgG1, K Iso Cntrl (cat. 57-4714) (left) or Pacific Blue® anti-human CD95 (DX2) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0959	Affinity Purified anti-human CD95 (Fas/APO-1)	N/A	N/A	FA FC IHC
13-0959	Biotin anti-human CD95 (Fas/APO-1)	N/A	N/A	FC
11-0959	FITC anti-human CD95 (Fas/APO-1)	488	518	FC
12-0959	PE anti-human CD95 (Fas/APO-1)	488	575	FC
15-0959	PE-Cy5 anti-human CD95 (Fas/APO-1)	488	670	FC
17-0959	Allophycocyanin (APC) anti-human CD95 (Fas/APO-1)	633	660	FC
25-0959	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD95 (Fas/APO-1)	488	760	FC
57-0959	Pacific Blue® anti-human CD95 (Fas/APO-1)	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DX2 monoclonal antibody reacts with human CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. DX2 does not block binding of EOS9.1, another antibody specific for human CD95.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This DX2 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This DX2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

Related Products

Cat. 57-4714 Pacific Blue® Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.