Product Information

Contents: Pacific Blue® anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)
Catalog Number: 57-0900
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS51
Isotype: Mouse IgG2a, κ

Staining of rat splenocytes with 0.25 μg of Pacific Blue® Mouse IgG2a, K Iso Cntrl (cat. 57-4724) (open histogram) or Pacific Blue® anti-mouse/rat CD90.1 (HIS51) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0900	Affinity Purified anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	N/A	N/A	FC IH/F
13-0900	Biotin anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	N/A	N/A	FC
11-0900	FITC anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	518	FC
12-0900	PE anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	575	FC
15-0900	PE-Cy5 anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	670	FC
17-0900	APC anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	633	660	FC
25-0900	Phycoerythrin-Cy7 (PE-Cy7) anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	760	FC
57-0900	Pacific Blue® anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)	402	421	FC
45-0900	PerCP-Cy5.5 anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)	488	690	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HIS51 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This HIS51 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kroese, F. G., N. K. de Boer, et al. 1995. Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues. Cell Immunol 162(2): 185-93.
Hosseinzadeh, H. and I. Goldschneider 1993. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol 150(5): 1670-9.
Ishizu, A., H. Ishikura, et al. 1995. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int Immunol 7(12): 1939-47.
Hermans, M. H. and D. Opstelten 1991. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem 39(12): 1627-34.

Related Products

Cat. 57-4724 Pacific Blue® Mouse IgG2a, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.