Product Information

Contents: Pacific Blue® anti-human CD45 (LCA)
Catalog Number: 57-0459
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI30
Isotype: Mouse IgG1, κ
HLDA No.: IV N816

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0459	Affinity Purified anti-human CD45 (LCA)	N/A	N/A	FC IHC
13-0459	Biotin anti-human CD45 (LCA)	N/A	N/A	FC
11-0459	FITC anti-human CD45 (LCA)	488	518	FC
12-0459	PE anti-human CD45 (LCA)	488	575	FC
15-0459	PE-Cy5 anti-human CD45 (LCA)	488	670	FC
17-0459	Allophycocyanin (APC) anti-human CD45 (LCA)	633	660	FC
19-0459	Cy5 anti-human CD45 (LCA)	633	670	FC
25-0459	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45 (LCA)	488	760	FC
35-0459	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD45 (LCA)	488	690	FC
57-0459	Pacific Blue® anti-human CD45 (LCA)	402	421	FC
27-0459	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD45 (LCA) (APC-Cy7 Equivalent)	633	779	FC
45-0459	PerCP-Cy5.5 anti-human CD45 (LCA) (PE-Cy5.5 replacement)	488	690	FC
93-0459	eFluor^TM 605^NC anti-human CD45 (LCA)	350-500	605	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI30 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 57-4714 Pacific Blue® Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.