Product Information

Contents: Pacific Blue® anti-human CD45RA
Catalog Number: 57-0458
Sizes: 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906

Staining of normal human peripheral blood cells with 1.0 μg of Pacific Blue® Mouse IgG2b Iso Cntrl (cat. 57-4732) (open histogram) or 1.0 μg of Pacific Blue® anti-human CD45RA (HI100) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0458	FITC anti-human CD45RA	488	518	FC
12-0458	PE anti-human CD45RA	488	575	FC
13-0458	Biotin anti-human CD45RA	N/A	N/A	FC
14-0458	Affinity Purified anti-human CD45RA	N/A	N/A	FC IHC
15-0458	PE-Cy5 anti-human CD45RA	488	670	FC
17-0458	Allophycocyanin (APC) anti-human CD45RA	633	660	FC
25-0458	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45RA	488	760	FC
45-0458	PerCP-Cy5.5 anti-human CD45RA	488	690	FC
57-0458	Pacific Blue® anti-human CD45RA	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4⁺ peripheral T lymphocytes, CD8⁺ peripheral T lymphocytes, and B cells. The CD45RA⁺ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HI100 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This HI100 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 57-4732 Pacific Blue® Mouse IgG2b Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.