Contents: Pacific BlueŽ anti-human CD45RA
Catalog Number: 57-0458
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI100
Isotype: Mouse IgG2b, κ
HLDA No.: IV N906
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Staining of normal human peripheral blood cells with 1.0 μg of Pacific BlueŽ Mouse IgG2b Iso Cntrl (cat. 57-4732) (open histogram) or 1.0 μg of Pacific BlueŽ anti-human CD45RA (HI100) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4
+ peripheral T lymphocytes, CD8
+ peripheral T lymphocytes, and B cells. The CD45RA
+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This HI100 antibody has been reported for use in flow cytometric analysis.
The Pacific BlueŽ emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This HI100 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
57-4732 Pacific BlueŽ Mouse IgG2b Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart