Product Information

Contents: Pacific Blue® anti-mouse/human CD45R (B220)
Catalog Number: 57-0452
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RA3-6B2
Isotype: Rat IgG2a, κ
HLDA No.: N/A

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (left) or 0.5 µg Pacific Blue anti-mouse/human CD45R (B220)(RA3-6B2) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0452	Affinity Purified anti-mouse/human CD45R (B220)	N/A	N/A	FC IH/F IHC(Paraffin) IP
16-0452	Functional Grade* Purified anti-mouse/human CD45R (B220)	N/A	N/A	FA FC
13-0452	Biotin anti-mouse/human CD45R (B220)	N/A	N/A	FC
11-0452	FITC anti-mouse/human CD45R (B220)	488	518	FC
12-0452	PE anti-mouse/human CD45R (B220)	488	575	FC
15-0452	PE-Cy5 anti-mouse/human CD45R (B220)	488	670	FC
17-0452	APC anti-mouse/human CD45R (B220)	633	660	FC
19-0452	Cy5 anti-mouse/human CD45R (B220)	633	670	FC
25-0452	PE-Cy7 anti-mouse/human CD45R (B220)	488	760	FC
30-0452	May be discontinued; see superior product Alexa Fluor® 700 Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse/human CD45R (B220) (Alexa Fluor® 700 Equivalent)	633	690	FC
35-0452	PE-Cy5.5 anti-mouse/human CD45R (B220)	488	690	FC
36-0452	Functional Grade* Biotin anti-mouse/human CD45R (B220)	N/A	N/A	FC
51-0452	Alexa Fluor® 647 anti-mouse/human CD45R (B220)	633	668	FC
53-0452	Alexa Fluor® 488 anti-mouse/human CD45R (B220)	488	519	FC
56-0452	Alexa Fluor® 700 anti-mouse/human CD45R (B220) (APC-Cy5.5 Equivalent)	633	723	FC
57-0452	Pacific Blue® anti-mouse/human CD45R (B220)	402	421	FC
27-0452	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse/human CD45R (B220) (APC-Cy7 Equivalent)	633	779	FC
45-0452	PerCP-Cy5.5 anti-mouse/human CD45R (B220)	488	690	FC
93-0452	eFluor^TM 605^NC anti-mouse/human CD45R ( B220)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RA3-6B2 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Morse, H. C. d., W. F. Davidson, et al. 1982. Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 129(6): 2612-5.

Coffman, R. L. 1982. Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev. 69: 5-23.

Schuhmann B, Dietrich A, et al. 2005. A role for brain-derived neurotrophic factor in B cell development. J Neuroimmunol. 163(1-2):15-23. (IHC frozen, PubMed)

Monteith CE, Chelack BJ, et al. 1996. Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues. Can J Vet Res. 60(3):193-8. (IHC frozen and paraffin, PubMed)

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Cat. 57-4321 Pacific Blue® Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.