Product Information

Contents: Pacific Blue® anti-human CD8
Catalog Number: 57-0086
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT8 (OKT-8)
Isotype: Mouse IgG2a

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (left) or Pacific Blue anti-human CD8 (OKT8) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0086	Fluorescein isothiocyanate (FITC) anti-human CD8	488	518	FC
12-0086	Phycoerythrin (PE) anti-human CD8	488	575	FC
13-0086	Biotin anti-human CD8	N/A	N/A	FC
14-0086	Affinity Purified anti-human CD8	N/A	N/A	FC
16-0086	Functional Grade* Purified anti-human CD8	N/A	N/A	FC
17-0086	Allophycocyanin (APC) anti-human CD8	633	660	FC
51-0086	Alexa Fluor® 647 anti-human CD8	633	668	FC
53-0086	Alexa Fluor® 488 anti-human CD8	488	519	FC
56-0086	Alexa Fluor® 700 anti-human CD8 (APC-Cy5.5 Equivalent)	633	723	FC
57-0086	Pacific Blue® anti-human CD8	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Kay HD, Horwitz DA. 1980. Evidence by reactivity with hybridoma antibodies for a probable myeloid origin of peripheral blood cells active in natural cytotoxicity and antibody-dependent cell-mediated cytotoxicity. J Clin Invest. 66(4):847-51.

Sayos J, Wu C, Morra M, Wang N, Zhang X, Allen D, van Schaik S, Notarangelo L, Geha R, Roncarolo MG, Oettgen H, De Vries JE, Aversa G, Terhorst C.The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM. Nature. 1998 Oct 1;395(6701):462-9. (OKT8, IP, PubMed)

Thomas Y, Sosman J, Irigoyen O, Friedman SM, Kung PC, Goldstein G, Chess L. 1980. Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative T-T interactions in the immunoregulation of B cell differentiation. J Immunol. 125(6):2402-8.

Campanelli R, Palermo B, Garbelli S, Mantovani S, Lucchi P, Necker A, Lantelme E, Giachino C. 2002. Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation. Int Immunol. 14(1):39-44.

Related Products

Cat. 11-0088    FITC anti-human CD8 (CD8a; alpha chain) (clone RPA-T8)
Cat. 12-0088    PE anti-human CD8 (CD8a; alpha chain) (clone RPA-T8)
Cat. 14-0088    Affinity Purified anti-human CD8 (CD8a; alpha chain) (clone RPA-T8)
Cat. 15-0088    PE-Cy5 anti-human CD8 (CD8a; alpha chain) (clone RPA-T8)
Cat. 17-0088    APC anti-human CD8 (CD8a; alpha chain) (clone RPA-T8)
Cat. 11-0089    FITC anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 12-0089    PE anti-human CD8 (CD8a; alpha chain) (clone HIT8a)
Cat. 13-0089    Biotin anti-human CD8 (CD8a; alpha chain) (clone HIT8a)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.