Product Information

Contents: Pacific Blue® anti-human CD4
Catalog Number: 57-0048
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT4 (OKT-4)
Isotype: Mouse IgG2b

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (left) or Pacific Blue® anti-human CD4 (OKT4) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0048	Affinity Purified anti-human CD4	N/A	N/A	FC IP
16-0048	Functional Grade* Purified anti-human CD4	N/A	N/A	FC IP
13-0048	Biotin anti-human CD4	N/A	N/A	FC
11-0048	Fluorescein isothiocyanate (FITC) anti-human CD4	488	518	FC
12-0048	Phycoerythrin (PE) anti-human CD4	488	575	FC
17-0048	Allophycocyanin (APC) anti-human CD4	633	660	FC
35-0048	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD4	488	690	FC
51-0048	Alexa Fluor® 647 anti-human CD4	633	668	FC
53-0048	Alexa Fluor® 488 anti-human CD4	488	519	FC
56-0048	Alexa Fluor® 700 anti-human CD4 (APC-Cy5.5 Equivalent)	633	723	FC
57-0048	Pacific Blue® anti-human CD4	402	421	FC
45-0048	PerCP-Cy5.5 anti-human CD4	488	690	FC
93-0048	eFluor^TM 605^NC anti-human CD4	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Reinherz EL, Kung PC, Goldstein G, Schlossman SF. 1979. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci. 76(8): 4061-5.

Bour S, Boulerice F, Wainberg MA. Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1. J Virol. 1991 Dec;65(12):6387-96. (OKT4, IP, PubMed)

Related Products

Cat. 11-0049    FITC anti-human CD4 (clone RPA-T4)
Cat. 12-0049    PE anti-human CD4 (clone RPA-T4)
Cat. 13-0049    Biotin anti-human CD4 (clone RPA-T4)
Cat. 14-0049    Affinity Purified anti-human CD4 (clone RPA-T4)
Cat. 15-0049    PE-Cy5 anti-human CD4 (clone RPA-T4)
Cat. 16-0049    Functional Grade Purified anti-human CD4 (clone RPA-T4)
Cat. 17-0049    APC anti-human CD4 (clone RPA-T4)
Cat. 25-0049    PE-Cy7 anti-human CD4 (clone RPA-T4)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.