Product Information

Contents: Pacific Blue® anti-human CD3
Catalog Number: 57-0038
Sizes: 25 tests, 100 tests, 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UCHT1
Isotype: Mouse IgG1, κ
HLDA No.: III 471

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and 1.0 μg of Pacific Blue® Mouse IgG1, K Iso Cntrl (cat. 57-4714) (left) or Pacific Blue® anti-human CD3 (UCHT1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0038	Affinity Purified anti-human CD3	N/A	N/A	FA FC IH/F
16-0038	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
13-0038	Biotin anti-human CD3	N/A	N/A	FC
11-0038	FITC anti-human CD3	488	518	FC
12-0038	PE anti-human CD3	488	575	FC
15-0038	PE-Cy5 anti-human CD3	488	670	FC
17-0038	APC anti-human CD3	633	660	FC
25-0038	PE-Cy7 anti-human CD3	488	760	FC
56-0038	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
57-0038	Pacific Blue® anti-human CD3	402	421	FC
27-0038	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD3 (APC-Cy7 Equivalent)	633	779	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This UCHT1 antibody has been reported for use in flow cytometric analysis.

The Pacific Blue® emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral bloof cells. This can be used at 20 μl (1.0 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 57-0032    Pacific Blue® anti-mouse CD3 (CD3e, epsilon subunit) (clone 17A2)
Cat. 57-0037    Pacific Blue® anti-human CD3 (clone OKT3)
Cat. 57-4714    Pacific Blue® Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.