Contents: Pacific BlueŽ anti-human CD1a
Catalog Number: 57-0019
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI149
Isotype: Mouse IgG1, κ
HLDA No.: V 5T CD01.01
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Staining of MOLT-4 cells with buffer (autofluorescence)(left) or Pacific Blue anti-human CD1a (HI149) (right). Total viable cells were used for analysis.
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Description
The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the β2-microglobulin and is thought to play a role in antigen presentation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This HI149 antibody has been reported for use in flow cytometric analysis.
The Pacific BlueŽ emits at 456 nm and can be excited with the Violet laser. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This HI149 antibody has been pre-titrated and tested by flow cytometric analysis of MOLT-4 cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
57-4714 Pacific BlueŽ Mouse IgG1, K Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart