Product Information

Contents: Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1, Sca1)
Catalog Number: 56-5981
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: D7
Isotype: Rat IgG2a, κ

Staining of 3-day ConA-activated C57Bl/6 splenocytes with 0.25 μg of Alexa Fluor® 700 Rat IgG2a Iso Cntrl (cat. 56-4321) (open histogram) or 0.25 μg of Alexa Fluor® 700 anti-mouse Sca-1 (D7) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5981	Affinity Purified anti-mouse Ly-6A/E (Sca-1, Sca1)	N/A	N/A	FC IP WB
16-5981	Functional Grade* Purified anti-mouse Ly-6A/E (Sca-1, Sca1)	N/A	N/A	FC
13-5981	Biotin anti-mouse Ly-6A/E (Sca-1, Sca1)	N/A	N/A	FC
11-5981	FITC anti-mouse Ly-6A/E (Sca-1, Sca1)	488	518	FC
12-5981	PE anti-mouse Ly-6A/E (Sca-1, Sca1)	488	575	FC
15-5981	PE-Cy5 anti-mouse Ly-6A/E (Sca-1, Sca1)	488	670	FC
17-5981	APC anti-mouse Ly-6A/E (Sca-1, Sca1)	633	660	FC
25-5981	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse Ly-6A/E (Sca-1, Sca1)	488	760	FC
35-5981	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6A/E (Sca-1, Sca1)	488	690	FC
56-5981	Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1, Sca1) (APC-Cy5.5 Equivalent)	633	723	FC
45-5981	PerCP-Cy5.5 anti-mouse Ly-6A/E (Sca-1, Sca1)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This D7 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This D7 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ortega, G., P. E. Korty, et al. (1986). "Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity." J Immunol 137(10): 3240-6.
Malek, T. R., G. Ortega, et al. (1986). "Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies." J Exp Med 164(3): 709-22.

Related Products

Cat. 56-4321 Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.