Product Information

Contents: Alexa Fluor® 700 anti-mouse Ly-6G (Gr-1, Gr1)
Catalog Number: 56-5931
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RB6-8C5
Isotype: Rat IgG2b, κ

Staining of BALB/c bone marrow cells with staining buffer (autofluorescence) (open histogram) or 0.06 μg of Alexa Fluor® 700 anti-mouse Gr-1 (RB6-8C5) (colored histogram). Cells in the large scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5931	FITC anti-mouse Ly-6G (Gr-1, Gr1)	488	518	FC
12-5931	PE anti-mouse Ly-6G (Gr-1, Gr1)	488	575	FC
13-5931	Biotin anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FC
14-5931	Affinity Purified anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FA FC IH/F IHC(Paraffin) IP WB
15-5931	PE-Cy5 anti-mouse Ly-6G (Gr-1, Gr1)	488	670	FC
16-5931	Functional Grade* Purified anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FA FC
17-5931	Allophycocyanin (APC) anti-mouse Ly-6G (Gr-1, Gr1)	633	660	FC
19-5931	Cy5 anti-mouse Ly-6G (Gr-1, Gr1)	633	670	FC
25-5931	PE-Cy7 anti-mouse Ly-6G (Gr-1, Gr1)	488	760	FC
27-5931	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse Ly-6G (Gr-1, Gr1) (APC-Cy7 Equivalent)	633	779	FC
35-5931	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6G (Gr-1, Gr1)	488	690	FC
36-5931	Functional Grade* Biotin anti-mouse Ly-6G (Gr-1, Gr1)	N/A	N/A	FC
45-5931	PerCP-Cy5.5 anti-mouse Ly-6G (Gr-1, Gr1)	488	690	FC
51-5931	Alexa Fluor® 647 anti-mouse Ly-6G (Gr-1, Gr1)	633	668	FC
53-5931	Alexa Fluor® 488 anti-mouse Ly-6G (Gr-1, Gr1)	488	519	FC
56-5931	Alexa Fluor® 700 anti-mouse Ly-6G (Gr-1, Gr1) (APC-Cy5.5 Equivalent)	633	723	FC
57-5931	Pacific Blue® anti-mouse Ly-6G (Gr-1, Gr1)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G and Ly-6C, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This RB6-8C5 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hestdal, K., F. W. Ruscetti, et al. (1991). "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." J Immunol 147(1): 22-8.
Fleming, T. J., M. L. Fleming, et al. (1993). "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." J Immunol 151(5): 2399-408.(RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C; PubMed)
Sasmono, R.T., A. Ehrnsperger, et al. (2007). "Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1." J Leukocyte Biol 82: 111-23.(RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C; Pubmed)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.