Product Information

Contents: Alexa Fluor® 700 anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) (APC-Cy5.5 Equivalent)
Catalog Number: 56-5821
Sizes: 25 ug, 50 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: Avas12a1
Isotype: Rat IgG2a, κ

Staining of bEND3 cells with Alexa Fluor® 700 Rat IgG2a Iso Cntrl (cat. 56-4321) (open histogram) or 0.5 μg of Alexa Fluor® 700 anti-mouse Flk-1 (Avas12a1) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5821	Affinity Purified anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	N/A	N/A	FC IHC WB
13-5821	Biotin anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	N/A	N/A	FC
12-5821	PE anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	488	575	FC
17-5821	Allophycocyanin (APC) anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	633	660	FC
56-5821	Alexa Fluor® 700 anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) (APC-Cy5.5 Equivalent)	633	723	FC
57-5821	Pacific Blue® anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	402	421	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This Avas12a1 antibody has been tested by flow cytometric analysis of bEND3 endothelial cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kataoka, H., N. Takakura, et al. (1997)."Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells." Dev Growth Differ 39(6): 729-40.

Related Products

Cat. 56-4321 Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.