Alexa Fluor® 700 anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) (APC-Cy5.5 Equivalent)

RUO: For Research Use Only

mouse Flk-1 antibody, Alexa Fluor® 700 conjugate

Staining of bEND3 cells with Alexa Fluor® 700 Rat IgG2a Iso Cntrl (cat. 56-4321) (open histogram) or 0.5 μg of Alexa Fluor® 700 anti-mouse Flk-1 (Avas12a1) (colored histogram). Total viable cells were used for analysis.

Contents: Alexa Fluor® 700 anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) (APC-Cy5.5 Equivalent)
Catalog Number: 56-5821
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Clone: Avas12a1
Host/Isotype: Rat IgG2a, κ
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
56-5821-80	25 ug	To view relevant pricing, please select your country and resume browsing.
56-5821-81	50 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
56-4321	Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-5821	PE anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	488	575	FC	RUO
13-5821	Biotin anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	N/A	N/A	FC	RUO
14-5821	Affinity Purified anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	N/A	N/A	FC IHC WB	RUO
17-5821	Allophycocyanin (APC) anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	633	660	FC	RUO
56-5821	Alexa Fluor® 700 anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73) (APC-Cy5.5 Equivalent)	633	723	FC	RUO
57-5821	Pacific Blue® anti-mouse Flk-1 (CD309, VEGF-R2, VEGFR2, Ly-73)	402	421	FC	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This Avas12a1 antibody has been tested by flow cytometric analysis of bEND3 endothelial cell line. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kataoka, H., N. Takakura, et al. (1997)."Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells." Dev Growth Differ 39(6): 729-40.