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Anti-Mouse CD309 (FLK1) Alexa Fluor® 700

Also known as: Flk-1, VEGF-R2, VEGFR2, Ly-73, Ly73, KDR

RUO: For Research Use Only
mouse Flk-1 antibody, Alexa Fluor® 700 conjugateStaining of bEND.3 cell line with Rat IgG2a κ Isotype Control Alexa Fluor® 700 (cat. 56-4321) (open histogram) or 0.5 µg of Anti-Mouse CD309 (FLK1) Alexa Fluor® 700 (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD309 (FLK1) Alexa Fluor® 700
Catalog Number: 56-5821
Concentration: 0.2 mg/ml
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: Avas12a1
Host/Isotype: Rat IgG2a, κ
 
Product Options
Cat. No. Size Price Add Qty to Cart
56-5821-80 25 ug
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56-5821-81 50 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
56-4321 Rat IgG2a K Isotype Control Alexa Fluor® 700
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
12-5821 Anti-Mouse CD309 (FLK1) PE 488 575 FC RUO
13-5821 Anti-Mouse CD309 (FLK1) Biotin N/A N/A FC RUO
14-5821 Anti-Mouse CD309 (FLK1) Purified N/A N/A FC IHC WB RUO
17-5821 Anti-Mouse CD309 (FLK1) APC 633 660 FC RUO
56-5821 Anti-Mouse CD309 (FLK1) Alexa Fluor® 700 633 723 FC RUO
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
This Avas12a1 antibody has been tested by flow cytometric analysis of bEND3 endothelial cell line. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References

Kataoka, H., N. Takakura, et al. (1997)."Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells." Dev Growth Differ 39(6): 729-40.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.