Product Information

Contents: Alexa Fluor® 700 Rat IgG2a Isotype Control
Catalog Number: 56-4321
Sizes: 25 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
10-4321	May be discontinued; see superior product APC-Alexa Fluor® 750 Allophycocyanin-Cy7 (APC-Cy7) Rat IgG2a Isotype Control	633	760	FC
11-4321	FITC Rat IgG2a Isotype Control	488	518	FC
12-4321	PE Rat IgG2a Isotype Control	488	575	FC
13-4321	Biotin Rat IgG2a Isotype Control	N/A	N/A	FC
14-4321	Affinity Purified Rat IgG2a Isotype Control	N/A	N/A	FC
15-4321	PE-Cy5 Rat IgG2a Isotype Control	488	670	FC
16-4321	Functional Grade* Purified Rat IgG2a Isotype Control	N/A	N/A	FC
17-4321	APC Rat IgG2a Isotype Control	633	660	FC
25-4321	Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control	488	760	FC
27-4321	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2a Isotype Control (APC-Cy7 Equivalent)	633	779	FC
30-4321	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) Rat IgG2a Isotype Control (Alexa Fluor® 700 Equivalent)	633	690	FC
35-4321	PE-Cy5.5 Rat IgG2a Isotype Control	488	690	FC
45-4321	PerCP-Cy5.5 Rat IgG2a Isotype Control	488	690	FC
51-4321	Alexa Fluor® 647 Rat IgG2a Isotype Control	633	668	FC
53-4321	Alexa Fluor® 488 Rat IgG2a Isotype Control	488	519	FC
56-4321	Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4321	Pacific Blue® Rat IgG2a Isotype Control	402	421	FC
72-4321	Phycoerythrin (PE) Set Rat IgG2a Isotype Control	488	575	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal rat IgG2a, κ is useful as an isotype control immunoglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Alexa Fluor® 700 Rat IgG2a Iso Cntrl has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at the same concentration as the experimental antibody.

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.